补托坐浴方对大鼠肛周脓肿术后创面愈合机制的Label-free蛋白质组学研究  

Exploring the Effect of Butuo Fumigation on Postoperative Wound Healing of Perianal Abscess in Rats Using Label-free based Quantitative Proteomics Analysis

作　　者：陈铭 陈兴华[1] 孟凡宇 俞婷 Chen Ming;Chen Xinghua;Meng Fanyu;Yu Ting(Jinshan Hospital of Fudan University,Shanghai 200540,China)

机构地区：[1]复旦大学附属金山医院,上海200540

出　　处：《中国中医急症》2024年第7期1169-1173,共5页Journal of Emergency in Traditional Chinese Medicine

基　　金：上海市金山区卫生健康专项项目资助(JSKJKTZY-2021-04,KTZY-2021-05)。

摘　　要：目的观察补托坐浴方对肛周脓肿术后模型大鼠创面愈合的影响,同时采用非标记定量(Label-free)蛋白质组学分析探寻其可能的作用机制。方法应用无特定病原体级SD大鼠建立肛周脓肿术后创面模型,随机分为对照组、模型组和补托坐浴方治疗组,连续治疗14d后,观察大鼠的创面生长情况,采用HE染色观察创面组织病理变化;通过Label-free蛋白质组学分析对照组、模型组、补托坐浴方治疗组大鼠术后创面组织差异蛋白。结果补托坐浴方治疗组胶原形成情况和表皮生长情况优于模型组和对照组(P<0.05)。蛋白质组学分析结果显示,模型组、对照组、补托坐浴方治疗组中共有11个差异表达蛋白,抗酒石酸酸性磷酸酶5(TRAP/ACP5)在3组中同时存在;且与模型组、对照组相比,补托坐浴方治疗组ACP5呈下降趋势。结论补托坐浴方治疗组可抑制炎症因子,可能通过调节p38/JNKMAPK信号通路的表达促进大鼠肛周脓肿术后创面愈合。Objective:To observe the effect of Butuo fumigation on postoperative wound healing in rats with perianal abscess,and to explore the possible mechanism of action by label-free proteomic analysis.Methods:A postoperative wound model of perianal abscess was established using Specific Pathogen Free(SPF)Sprague-Dawley(SD)rats.The rats were randomly divided into three groups:the control group,the model group,and the Butuo fumigation group.After continuous treatment for 14 days,the growth of the wounds was observed.Hematoxylin-eosin(HE)staining was used to observe the histopathological changes in the wound tissues.Label-free proteomics analysis was employed to identify differential proteins in the postoperative wound tissues from the control group model group,and the Butuo fumigation group.Results:Compared with the control group and the model group,the collagen formation and epidermal growth of the Butuo fumigation group were beter(P<0.05).The proteomics analysis revealed 11 differentially expressed proteins among the three groups.Tartrate-resistant Acid Phosphatase 5(TRAP/ACP5)was present in all three groups;notably,ACP5 levels in the Butuo fumigation group showed a downward trend compared with the model and control groups.Conclusion:Butuo fumigation can inhibit inflammatory factors and promote the postoperative wound healing of perianal abscess in rats by regulating the expression of p38/JNK MAPK signaling pathway.

关 键 词：肛周脓肿 蛋白质组学 抗酒石酸酸性磷酸酶5 炎症 MAPK信号通路 大鼠 

分 类 号：R285.5[医药卫生—中药学]