瑶药小钻的特征图谱及其安五脂素含量和抗炎活性研究  

Study on characteristic chromatogram of Yao medicine Kadsura longipedunculata and its anwulignan content and anti-inflammatory activity

作　　者：唐炳兰[1] 高微 赵成坚[1] 欧春丽 候小利 陈路[1] 莫单丹[1] TANG Binglan;GAO Wei;ZHAO Chengjian;OU Chunli;HOU Xiaoli;CHEN Lu;MO Dandan(National Traditional Chinese Medicine Inheritance and Innovation Center,Guangxi Botanical Garden of Medicinal Plant/Guangxi Key Laboratory of Medicinal Resource Protection and Genetic Improvement/Guangxi Zhuang Autonomous Region Traditional Chinese Medicine Resources Intelligent Creation Engineering Research Center,Nanning 530023,China;Guangxi Zhuang Autonomous Region Drug Administration,Nanning 530029,China)

机构地区：[1]广西壮族自治区药用植物园国家中医药传承创新中心/广西药用资源保护与遗传改良重点实验室/广西壮族自治区中药资源智慧创制工程研究中心,南宁530023 [2]广西壮族自治区药品监督管理局,南宁530029

出　　处：《中国药房》2024年第14期1727-1731,共5页China Pharmacy

基　　金：广西壮族自治区中医药管理局自筹经费科研课题(No.GXZYA20220005);广西壮族自治区药品监督管理局药品安全科研项目。

摘　　要：目的 建立瑶药小钻特征图谱及其主要成分安五脂素含量测定方法,并评价安五脂素抗炎活性。方法 采用高效液相色谱法,以乙腈-0.5%磷酸溶液为流动相进行梯度洗脱,采用《中药色谱指纹图谱相似度评价系统(2012版)》建立小钻药材的特征图谱和进行相似度评价,并测定小钻中安五脂素含量。以脂多糖诱导的RAW264.7巨噬细胞为炎症细胞模型,考察安五脂素对细胞上清液中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、IL-6水平的影响。结果 10批小钻样品的特征图谱相似度为0.901~0.994;标定了9个共有峰,指认了3个成分,分别为长南五味子素E、南五味子内酯A、安五脂素;安五脂素的含量为(0.72±0.05)~(1.21±0.03)mg/g(n=3)。0.125~0.5μg/mL的安五脂素可显著降低炎症模型细胞上清液中TNF-α、IL-1β、IL-6水平(P<0.05或P<0.01)。结论 建立了小钻药材HPLC特征图谱及其安五脂素含量测定方法;安五脂素可能是小钻药材发挥抗炎作用的有效成分。OBJECTIVE To establish characteristic chromatogram of Yao medicine Kadsura longipedunculata and the method for the content determination of its main component anwulignan,and evaluate the anti-inflammatory activity of anwulignan.METHODS HPLC method was performed with acetonitrile-0.5% phosphoric acid solution as the mobile phase for gradient elution.The characteristic chromatogram of K.longipedunculata was established and similarity was evaluated by Similarity Evaluation System for Chromatographic Fingerprint of TCM(2012 edition).The content of anwulignan in K.longipedunculata was determined.Lipopolysaccharide induced RAW264.7 macrophages were selected as inflammatory cell model to investigate the effects of anwulignan on the levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β) and IL-6.RESULTS The similarities of characteristic chromatogram for 10 batches of K.longipedunculata ranged 0.901-0.994,and 9 common peaks were determined;3components were identified,such as changnan schisantherin E,kadsulactone A,anwulignan.The contents of anwulignan were(0.72±0.05)-(1.21±0.03) mg/g(n=3).Anwulignan of 0.125-0.5 μg/mL greatly decreased the levels of TNF-α,IL-1β and IL-6 in the supernatant of inflammatory model cells(P<0.05 or P<0.01).CONCLUSIONS HPLC characteristic chromatogram of K.longipedunculata and the method for the content determination of anwulignan are all established,and anwulignan may be the active ingredient of anti-inflammatory effect in K.longipedunculata.

关 键 词：瑶药小钻 HPLC 特征图谱 质量控制 安五脂素 抗炎作用 

分 类 号：R917[医药卫生—药物分析学] R285[医药卫生—药学]