超声协同SDS-Na_(2)CO_(3)提取丝素蛋白及高强韧纳米纤维膜的制备与细胞相容性研究  

Ultrasonic co-extraction of silk fibroin with SDS-Na_(2)CO_(3) and preparation/cytocompatibility of high-strength and tough nanofiber membranes

作　　者：牛佳杰 寿万 刘雍[1] NIU Jiajie;SHOU Wan;LIU Yong(Key Laboratory of Advanced Textile Composites,Ministry of Education,School of Textile Science and Engineering,Tiangong University,Tianjin,China;Department of Mechanical Engineering,University of Arkansas,Arkansas,the U.S.)

机构地区：[1]天津工业大学先进纺织复合材料教育部重点实验室,纺织科学与工程学院,天津 [2]阿肯色大学费耶特维尔分校机械工程系,阿肯色州美国

出　　处：《东华大学学报（自然科学版）》2024年第4期148-155,共8页Journal of Donghua University(Natural Science)

摘　　要：为解决提取过程中丝素蛋白极易受损的问题,提出以Na_(2)CO_(3)和表面活性剂SDS(十二烷基磺酸钠)的复合体系为脱胶剂,再结合超声处理的一体化天然蚕丝脱胶方案。采用该方案所得丝素蛋白纤维表面更加光洁平整,纤维损伤显著减少;相比传统的100℃碱液脱胶(30min完全脱胶),该方案在75℃下仅用10min即完成脱胶。以该方案所得脱胶纤维为原料,通过静电纺丝技术制备丝素蛋白纳米纤维膜,所得纤维膜经乙醇处理后,β折叠链片晶含量增至49.58%,展现出高达5.27MPa的断裂应力和123.49%的断裂应变。在5d的细胞培养试验中,丝素蛋白纳米纤维膜支持细胞的黏附、铺展和增殖,表现出良好的细胞相容性。这些研究成果将为制备高性能丝素蛋白纳米纤维膜提供解决方案,并有望推动丝素蛋白材料在生物医用领域的进一步应用和发展。Silk fibroin is highly susceptible to damage during the extraction process.To address this issue,this study proposes an integrated degumming solution that uses a composite system of Na_(2)CO_(3) and the surfactant SDS(sodium dodecyl sulfate),combined with ultrasonic treatment.Silk fibroin fibers treated with this method exhibit a smoother and more even surface,with markedly less fiber damage.Compared to the traditional 100℃ alkaline degumming(30 min complete degumming),this scheme completes degumming in only 10 min at 75℃.Nanofibrous membranes were prepared from these degummed fibers using electrospinning technology,and their β-sheet crystalline content increased to 49.58% after ethanol treatment,along with high tensile strength(5.27 MPa)and elongation at break(123.49%).Over a 5-day cell culture period,the membranes have supported cell adhesion,spreading and proliferation,demonstrating excellent cytocompatibility.These research findings provide a novel solution for the production of high-performance silk fibroin nanofibrous membranes and are expected to advance the application and development of silk fibroin materials in the biomedical field.

关 键 词：丝素蛋白 脱胶 静电纺丝 细胞相容性 

分 类 号：TS141[轻工技术与工程—纺织材料与纺织品设计]