Proximate analyses,in vitro antioxidant activity and GC-MS analyses of fermented Pentaclethra macrophylla seeds  

作　　者：Ikechukwu Okoro Parker Joshua Innocent Okagu Christian Amah Nnamani Ikenna Nene Chiaka-Onyemeze Emeka Anaduaka David Obasi Prince Okoroh Daniel Orieke Victor Ogugua 

机构地区：[1]Department of Medical Biochemistry,College of Health Sciences,David Umahi Federal University of Health Sciences,Uburu,Nigeria [2]Department of Biochemistry,University of Nigeria,Nsukka,Nigeria [3]Department of Biochemistry,State University of Medical and Applied Sciences,Igbo-Eno,Nigeria [4]Department of Medical Laboratory Sciences,State University of Medical and Applied Sciences,Igbo-Eno,Nigeria

出　　处：《Life Research》2024年第3期2-8,共7页TMR生命研究

摘　　要：The present study assessed the proximate composition,in vitro antioxidant activity and GC-MS analyses of fermented Pentaclethra macrophylla seeds extract(FPMSE).The in vitro antioxidant activity was assessed using 2,2-diphenyl-1-picrylhydrazl(DPPH)while Gas Chromatography-Mass Spectrophotometry(GC-MS)technique was used to identify the volatile compounds in FPMSE.FPMSE revealed a dose dependent percentage inhibition of the extract on DPPH assay.Proximate composition of FPMSE constitutes respectively;ash(0.33±0.12%),protein(2.19±0.08%),fats and oil(80.60±0.00%),carbohydrate(16.88±0.00%)while moisture and fibre were not detected.However,the pulverised sample constitute respectively;ash(1.27±0.58%),protein(10.51±0.60%),moisture(8.27±0.12%),fats and oil(32.33±0.12%),fibre(7.27±0.12%)and carbohydrate(40.35±0.00%).GC-MS analyses revealed fourteen(14)fatty acids including Octadecanoic acid(1.95%),Benzyloxymethylimine(43.89%),2-Allylpent-4-enoic acid benzyl ester(26.25%),Hexadecanoic acid,methyl ester(1.09%),Oleic Acid(0.25%),9,12-Octadecadienoic acid,methyl ester(Omega-6-fatty acid)(1.52%).The bioactive compounds identified in FPMSE could contribute to the pharmacological properties of P.macrophylla seeds and hence,could be of considerable interest for the development of new drugs.

关 键 词：Pentaclethra macrophylla in vitro antioxidant GC-MS fatty acids 

分 类 号：R28[医药卫生—中药学]