Regulating CRISPR/Cas9 Using Streptavidin-Biotin Interactions  

作　　者：Wei Shen Wei Xiong Qianqian Qi Xingyu Liu Zhongpao Xie Yuanyuan Zhang Jinxuan Hou Tian Tian Xiang Zhou 

机构地区：[1]College of Chemistry and Molecular Sciences,Wuhan University,Wuhan,Hubei 430072,China [2]Department of Thyroid&Breast Surgery Zhongnan Hospitalof Wuhan University Wuhan,Hubei43001,Chin

出　　处：《Chinese Journal of Chemistry》2024年第12期1387-1393,共7页中国化学（英文版）

基　　金：the National Natural Science Foundation of China(Nos.22177089,21721005,92153303,22037004,22177088);the Fundamental Research Funds for the Central Universities(2042023kf0204);Translational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University(Grant No.ZNJC202309).

摘　　要：Comprehensive Summary Currently,CRISPR/Cas9 technology has found widespread applications across various domains.However,the utility of CRISPR/Cas9 is encumbered by issues pertaining to its reliability and safety,primarily stemming from the uncontrolled activity of the system.Therefore,the design and development of CRISPR/Cas9 systems with controllable activity is of paramount importance.Biotin,characterized by its small molecular weight,and streptavidin,distinguished by its substantial spatial steric hindrance,can be harnessed as an ideal OFF switch(termed a"bioactivity brake")due to their interaction characteristics.In this work,we present a strategy that employs the streptavidin-biotin interaction as a"brake system"for CRISPR/Cas9,effectively allowing for the shutdown of the enzymatic activity of CRISPR/Cas9.

关 键 词：Streptavidin-biotin CRISPR/Cas9 CRISPR-OFF Guide RNA 2'-OH acylation Gene technology DNA cleavage 

分 类 号：Q78[生物学—分子生物学]