丛枝菌根真菌提高入侵杂草南美蟛蜞菊对除草剂的耐受性  

Arbuscular mycorrhizal fungi enhance the capacity of invasive Sphagneticola trilobata to tolerate herbicides

作　　者：胡蝶 蒋欣琪 戴志聪 陈戴一 张雨 祁珊珊 杜道林 HU Die;JIANG Xin-Qi;DAI Zhi-Cong;CHEN Dai-Yi;ZHANG Yu;QI Shan-Shan;DU Dao-Lin(School of Agricultural Engineering,Jiangsu University,Zhenjiang,Jiangsu 212013,China;School of the Environment and Safety Engineering,Institute of Environment and Ecology,Jiangsu University,Zhenjiang,Jiangsu 212013,China;Jingjiang College,Jiangsu University,Zhenjiang,Jiangsu 212013,China)

机构地区：[1]江苏大学农业工程学院,江苏镇江212013 [2]江苏大学环境与安全工程学院,环境生态研究所,江苏镇江212013 [3]江苏大学京江学院,江苏镇江212013

出　　处：《植物生态学报》2024年第5期651-659,共9页Chinese Journal of Plant Ecology

基　　金：国家自然科学基金(32171509和32271587);江苏省自然科学基金(BK20211321)。

摘　　要：入侵杂草南美蟛蜞菊(Sphagneticola trilobata)的优势生长严重危害本土植物群落和生态系统的稳定性。近年来,化学防治依然是最主要的杂草防控手段。丛枝菌根真菌(AMF)作为一种菌根共生体,在宿主植物的生长和抵抗外界环境胁迫中起到重要的作用。该研究通过温室控制实验设置4种处理方式:对照组、只接种AMF、只喷施除草剂以及喷施除草剂并接种AMF,以验证AMF是否在入侵杂草南美蟛蜞菊响应除草剂中起到重要作用。结果显示:在草甘膦铵盐除草剂的胁迫下,南美蟛蜞菊的菌根侵染率、泡囊数以及菌根侵染丰度等级占比都显著上升;相比于只喷施除草剂处理,接种AMF显著增加南美蟛蜞菊的叶面积、地上生物量和根冠比,显著减少黄酮醇相对含量以及叶片损害数。首次发现与AMF的共生能缓解除草剂对入侵杂草南美蟛蜞菊的胁迫。因此,在杂草的化学防治过程中,与AMF的共生可能极大提高杂草对除草剂的抗性,可为入侵杂草的有效防控提供新的思考途径。Aims Invasive Sphagneticola trilobata seriously endangers the stability of local plant communities and ecosystems.Recently,chemical control is still the most important method for controlling it.Arbuscular mycorrhizal fungi(AMF)play an important role in the growth of host plants and their resistance to environmental stresses.Therefore,this study tested whether AMF played an important role in herbicide resistance of S.trilobata.Methods In this study,a greenhouse experiment was conducted,in which the stems of S.trilobata were subject to the following four treatments:control(CK),only inoculation of AMF,only application of herbicides(HC),and herbicide plus AMF(AMF+HC).Important findings The results showed that under the stress of glyphosate ammonium salt,the colonization rate,vesicle number and ratio of colonization abundance class increased significantly.Compared with herbicide application,AMF inoculation significantly increased the leaf area,above-ground biomass and root shoot ratio of S.trilobata,and significantly reduced the flavonol relative content and the damaged leaf number.This study found that the herbicide stress on invasive S.trilobata was alleviated because of the symbiosis with AMF.Therefore,AMF may greatly improve the resistance to chemical herbicides in this invasive forb.The results of this study may provide a new insight into the effective control of invasive weeds.

关 键 词：南美蟛蜞菊 丛枝菌根真菌 草甘膦铵盐除草剂 植物微生物互作 

分 类 号：S451[农业科学—植物保护]