大叶绣球艾薇塔组培快繁技术  

Study on tissue culture and rapid propagation of Hydrangea macrophylla cv.Ivetta

作　　者：陈双双 秦紫艺 邱帅 冯景 高凯 齐香玉 陈慧杰 邓衍明[1,2] CHEN Shuangshuang;QIN Ziyi;QIU Shuai;FENG Jing;GAO Kai;QI Xiangyu;CHEN Huijie;DENG Yanming(National Key Laboratory of Efficient Production of Forest Resources,Institute of Leisure Agriculture,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,Jiangsu;College of Horticulture,Nanjing Agricultural University,Nanjing 210008,Jiangsu;Hangzhou Landscaping Co.,Ltd.,Hangzhou 310009,Zhejiang)

机构地区：[1]江苏省农业科学院休闲农业研究所林木资源高效生产全国重点实验室,江苏南京210014 [2]南京农业大学园艺学院,江苏南京210008 [3]杭州市园林绿化股份有限公司,浙江杭州310009

出　　处：《浙江农业科学》2024年第7期1627-1633,共7页Journal of Zhejiang Agricultural Sciences

基　　金：国家自然科学基金(32371956);江苏省农业科技自主创新资金项目(CX(22)2035);江苏省种业振兴“揭榜挂帅”项目(JBGS[2021]097)。

摘　　要：为建立大叶绣球艾薇塔规模化繁殖和高效遗传转化体系,以艾薇塔带芽茎段为外植体,研究不同消毒方式、基本培养基类型、激素类型与浓度、移栽基质等因素对不定芽诱导、生根和移栽成活率的影响。结果表明,艾薇塔利用有效氯浓度为2%的NaClO消毒10 min,污染率较低;腋芽启动最适培养基为1/2 B5+2.0 mg·L^(-1)6-苄基腺嘌呤(6-BA);最适增殖培养基为B5+2.0 mg·L^(-1)6-BA;最佳生根培养基为1/2 B5+0.5 mg·L^(-1)IBA;无菌苗最适移栽基质为珍珠岩∶泥炭土=1∶1(V∶V)。研究初步建立了大叶绣球艾薇塔的组培快繁技术体系,可为其工厂化育苗和规模化生产提供技术支持。In order to establish a large-scale propagation and efficient genetic transformation system of Hydrangea macrophylla cv.Ivetta,the effects of different disinfection methods,basic medium types,hormone types and concentrations,transplanting substrate and other factors on adventitious bud induction,rooting and transplanting survival rate were studied by using Ivetta with bud shoots as explants.The results showed that Ivetta was disinfected by NaClO with an effective chlorine concentration of 2%for 10 min,and the contamination rate was low,and the optimal medium for axillary bud initiation was 1/2 B5+2.0 mg·L^(-1)6-BA;The optimal proliferation medium was B5+2.0 mg·L^(-1)6-BA;The optimal rooting medium was 1/2 B5+0.5 mg·L^(-1) IBA;The most suitable substrate for transplanting sterile seedlings was perlite∶peat soil=1∶1(V∶V).In this study,the tissue culture and rapid propagation technology system of Ivetta was preliminarily established,which could provide technical support for its factory seedling breeding and large-scale production.

关 键 词：大叶绣球 艾薇塔 组织培养 快速繁殖 

分 类 号：S682.1[农业科学—观赏园艺]