海滨木槿种子无菌萌发及壮苗的培养基筛选  

Screening of culture medium for aseptic germination and strengthening cultivation of Hibiscus hamabo

作　　者：魏馨[1] 李乾辰 周天培 温在扬 陈秋夏[1] 刘星 WEI Xin;LI Qianchen;ZHOU Tianpei;WEN Zaiyang;CHEN Qiuxia;LIU Xing(Zhejiang Institute of Subtropical Crops,Zhejiang Academy of Agricultural Sciences,Wenzhou 325005,Zhejiang;College of Life and Environmental Sciences,Wenzhou University,Wenzhou 325000,Zhejiang;Yueqing Bureau of Natural Resources and Planning,Wenzhou 325600,Zhejiang;Cangnan Forestry and Business Development Center,Wenzhou 325800,Zhejiang)

机构地区：[1]浙江省农业科学院浙江省亚热带作物研究所,浙江温州325005 [2]温州大学生命与环境科学学院,浙江温州325000 [3]乐清市自然资源和规划局,浙江温州325600 [4]苍南林业事业发展中心,浙江温州325800

出　　处：《浙江农业科学》2024年第7期1634-1638,共5页Journal of Zhejiang Agricultural Sciences

基　　金：浙江省“十四五”育种专项林木协作组课题“沿海防护林树种新品种选育”(2021C02070-6)。

摘　　要：海滨木槿是东南沿海地区防风护堤林的首选树种,也是瘠薄山地水保林的良好树种。无菌壮苗的获取是建立高效再生体系的前提,可为植物资源保护和新品种选育提供基础。该研究以MS为基本培养基,比较柠檬酸、活性炭、三碘苯甲酸(TIBA)、6-苄氨基腺嘌呤(6-BA)、萘乙酸(NAA)、水解酪蛋白、维生素H、叶酸、噻苯隆(TDZ)和寡糖·链蛋白等添加物对海滨木槿种子萌发以及壮苗培养的影响。结果显示,种子萌发最佳配方为MS+6-BA 0.5 mg·L^(-1)+NAA 0.1 mg·L^(-1)+水解酪蛋白0.1 g·L^(-1),萌发率从21.4%±7.8%提高到85.3%±9.1%;维生素、叶酸、柠檬酸的添加均未能促进种子的萌发。培养基MS+6-BA 0.1 mg·L^(-1)+寡糖·链蛋白0.1 g·L^(-1)+活性炭0.1%中的幼苗增殖系数最高,达7.0±2.6,且直立生长,茎枝较粗,叶片肥厚。值得注意的是,TDZ和NAA组合诱导出来的材料分化能力较强,可为后期海滨木槿诱导体胚成苗技术研究提供一定的参考。Hibiscus hamabo is not only a preferred species for windbreak forests in southeast coastal areas,but also a good species for water conservation forests in barren mountains.The acquisition of aseptic seedlings is a prerequisite for the establishment of efficient regeneration systems,which could provide a basis for plant resource conservation and new variety selection and breeding.In this study,MS was used as the basic medium to compare the effects of additives such as citric acid,activated carbon,triiodobenzoicacid(TIBA),6-benzylaminopurine(6-BA),naphthalene acetic acid(NAA),casein hydrolyzed,vitamin H,folic acid,thidiazuron(TDZ),oligosaccharidic plant activator protein,etc.on the seed germination as well as the aseptic seedling cultivation of Hibiscus hamabo.The optimum formulation for seed germination was MS+6-BA(0.5 mg·L^(-1))+NAA(0.1 mg·L^(-1))+hydrolyzed casein(0.1 g·L^(-1)),which increased the germination rate from 21.4%±7.8%to 85.3%±9.1%.Meanwhile,vitamin H,folic acid,and citric acid application failed to promote seed germination.Seedlings in medium of MS+6-BA(0.1 mg·L^(-1))+oligosaccharide chain protein(0.1 g·L^(-1))+activated carbon(0.1%)had the highest proliferation coefficient of 7.0±2.6 with thick stems,branches,and leaves.Notably,the combination of TDZ and NAA could induce the generation of Hibiscus hamabo with strong differentiation ability,which can provide a foundation for further developing the somatic embryo seedling technology of Hibiscus hamabo.

关 键 词：海滨木槿 配方筛选 6-BA 水解酪蛋白 寡糖·链蛋白 

分 类 号：S682[农业科学—观赏园艺]