不同尺寸形貌抑冰微纳米材料对细胞冷冻保护性能的影响  

Cryoprotective effects of deicing micro/nanomaterials with different sizes and shapes on cells

作　　者：许淼 宋玉 张静金秋 赵叶瑜 耿华曼 翁晓刚 刘忠华[1,2] 程金菊 颜廷胜 XU Miao;SONG Yu;ZHANG Jingjinqiu;ZHAO Yeyu;GENG Huaman;WENG Xiaogang;LIU Zhonghua;CHENG Jinju;YAN Tingsheng(Key Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province,Northeast Agricultural University,Harbin 150030,Heilongjiang,China;School of Life Science,Northeast Agricultural University,Harbin 150030,Heilongjiang,China;School of Food Science,Northeast Agricultural University,Harbin 150030,Heilongjiang,China)

机构地区：[1]东北农业大学黑龙江省动物细胞与遗传工程重点实验室,黑龙江哈尔滨150030 [2]东北农业大学生命科学学院,黑龙江哈尔滨150030 [3]东北农业大学食品科学学院,黑龙江哈尔滨150030

出　　处：《生物工程学报》2024年第7期2294-2307,共14页Chinese Journal of Biotechnology

基　　金：科技创新2030-重大项目(2023ZD0407503);黑龙江省优秀青年科学基金(YQ2023C020);中国博士后科学基金(2023MD734140)。

摘　　要：抑冰纳米材料被广泛研究以提高对细胞、组织或器官等的冷冻保存效率。不同组分、尺寸、形貌的抑冰纳米材料可有效抑制冰晶的形成和生长,从而减少对生物样品冻存的损伤。本研究通过水热法制备出不同尺寸的碳复合体微粒(carbon composite particles,CCPs)和特殊形貌的微纳米材料。结果显示随着碳复合体微粒粒径尺寸减小,细胞冻存保护效果增强。而相较于球形结构的碳复合体微粒,不对称纳米粒子(Janus nanoparticles)和纳米花(WSP nanoflower)材料对细胞冻存的保护作用明显增强。另外,将适当浓度微纳米抑冰材料与商品化冷冻保护剂联合使用,可进一步提高对细胞冷冻保护的效果。本文制备的微纳米防冻材料可有效提高细胞的冷冻保存效率,在生物医学研究和低温保存应用中具有巨大的潜力。Extensive studies have been conducted on deicing nanomaterials to improve the cryoprotective effects on cells,tissues,and organs.The nanomaterials with different composition,sizes,and shapes can inhibit the formation and growth of ice crystals,thereby reducing the damage to the cryopreserved samples.In this study,the carbon composite particles(CCPs)with different sizes and shapes were prepared by the hydrothermal method.The results demonstrated that the cryoprotective effect of CCPs enhanced with the decrease in particle size.Compared with spherical CCPs,Janus nanoparticles and WSP nanoflower with special shapes demonstrated improved protective effects on cryopreserved cells.In addition,the combination of deicing micro/nanomaterials at appropriate concentrations with commercial cryoprotectants exerted improved cryoprotective effects on cells.The prepared deicing micro/nanomaterials can improve cell cryopreservation,demonstrating great application potential in biomedical research and cryopreservation.

关 键 词：抑冰材料 细胞冻存 碳复合体微粒 纳米花 不对称纳米粒子 

分 类 号：TB383.1[一般工业技术—材料科学与工程] Q2-33[生物学—细胞生物学]