肉鹅坦布苏病毒病免疫程序初探  

Preliminary Study on Vaccination Procedure of Tembusu Virus Disease in Geese

作　　者：段娜 韦汉群 何晓露 张文丽[1] 杨凤仪 郝桂英[1,3] DUAN Na;WEI Hanqun;HE Xiaolu;ZHANG Wenli;YANG Fengyi;HAO Guiying(College of Animal Science,Xichang University,Xichang 615013,Sichuan,China;Xichang Agricultural and Rural Bureau,Xichang 615000,Sichuan,China;Panxi Laboratory of Animal Epidemic Disease Detection and Control of Sichuan,Xichang 615013,Sichuan,China)

机构地区：[1]西昌学院动物科学学院,四川西昌615013 [2]西昌市农业农村局,四川西昌615000 [3]攀西动物疫病检测与防控实验室,四川西昌615013

出　　处：《西昌学院学报（自然科学版）》2024年第2期11-16,共6页Journal of Xichang University(Natural Science Edition)

基　　金：西昌市农业科技创新项目(KJcx-2021-01)。

摘　　要：为了制定合理、有效的肉鹅坦布苏病毒病免疫程序,选取鸭坦布苏病毒病灭活疫苗(HB株)和弱毒疫苗(FX2010-180P株)对7日龄和14日龄浙东白鹅进行不同疫苗组合(7日龄灭活苗+21日龄灭活苗组(A组)、7日龄弱毒疫苗组(B组)、7日龄弱毒疫苗+21日龄灭活苗组(C组)、14日龄灭活苗+28日龄灭活苗组(D组)、14日龄弱毒疫苗组(E组)、14日龄弱毒疫苗+28日龄灭活苗组(F组)、未免疫组(G组))的试验,用双抗体夹心ELISA法检测免疫后不同时间的抗体含量。结果表明:弱毒疫苗免疫组产生抗体较其他组快,但抗体含量持续时间较短;灭活疫苗+灭活疫苗(A组和D组)抗体含量从免疫完成后第24~36天抗体含量均高于弱毒疫苗(B组和E组)和弱毒疫苗+灭活疫苗(C组和F组),抗体持续时间更长。建议肉鹅可在14日龄用坦布苏病毒病灭活疫苗进行首免,14d后再接种一次,可获得较高的抗体含量。In order to formulate a scientific and efficient vaccination procedure of Tembusu virus disease in meat geese,inactivated vaccine(HB strain)and attenuated vaccine(FX2010-180P strain)of duck Tembusu virus disease were se-lected to carry out different vaccine combinations(7-day-old inactivated vaccine+21-day-old inactivated vaccine group(group A),7-day-old attenuated vaccine group(group B),7-day-old attenuated vaccine+21-day-old inacti-vated vaccine group(group C),14-day-old inactivated vaccine+28-day-old inactivated vaccine group(group D),14-day-old attenuated vaccine group(group E),14-day-old attenuated vaccine+28-day-old inactivated vaccine group(group F),non-immunized group(group G))test on 7-day-old and 14-day-old Zhedong white geese in this study.Double antibody sandwich ELISA was used to detect the antibody content at different periods after vaccination.The re-sults showed that:attenuated vaccinated group produced antibodies faster than the other groups,but the antibody con-tent did not last long.The antibody contents of inactivated vaccine+inactivated vaccine(group A,group D)were higher than that of attenuated vaccine(group B,group E)and attenuated vaccine+inactivated vaccine(group C,group F)from day 24th to day 36th after vaccination completion,and the antibodies last longer.According to the results of this study,it is suggested that meat geese can be vaccinated with inactivated vaccine of Tembusu virus disease at 14-day-old,and then vaccinated again 14 days later to obtain higher antibody content.

关 键 词：坦布苏病毒 灭活疫苗(HB株) 弱毒疫苗(FX2010-180P株) 浙东白鹅 

分 类 号：S852.65[农业科学—基础兽医学]