早期脊髓电刺激对大鼠脊髓损伤后疼痛的镇痛机制研究  

Analgesic mechanism study of neuropathic pain relieved by early spinal cord stimulation in rats with spinal cord injury

作　　者：尹晶 孙思思 蔡猛 刘红军 金毅 YIN Jing;SUN Si-si;CAI Meng;LIU Hong-jun;JIN Yi(Department of Pain Medicine,Affiliated Jinling Hospital,Medical School of Nanjing University,Nanjing 210002,China)

机构地区：[1]南京大学医学院附属金陵医院疼痛科,南京210002

出　　处：《中国疼痛医学杂志》2024年第7期494-500,共7页Chinese Journal of Pain Medicine

基　　金：院管课题(YYZD2021004)。

摘　　要：目的:探究早期脊髓电刺激对大鼠脊髓损伤后神经病理性疼痛的疗效和脊髓背角内瞬时感受器电位香草酸受体1(transient receptor potential vanilloid 1,TRPV1)和C-C趋化因子配体2(C-C chemok-ine ligand 2,CCL2)表达的影响。方法:雌性SD大鼠64只按随机数字表法分为4组:空白对照组、假手术组、脊髓损伤组、脊髓损伤+电刺激组,每组16只。观察术前、术后7、14天机械刺激缩足反射阈值(mechanicalwithdrawalthreshold,MWT)和热缩足反射潜伏期(thermalwithdrawallatency,TWL)的变化,采用qPCR法和Western Blot法分别检测TRPV1和CCL2 mRNA及其蛋白表达,ELISA法检测炎性细胞因子IL-6、IL-1β和TNF-α含量。结果:与空白对照组和假手术组比较,术后7、14天脊髓损伤组大鼠50%MWT和TWL降低、TRPV1和CCL2转录和表达升高(P<0.05)、IL-6、IL-1β和TNF-α表达升高(P<0.001);与脊髓损伤组相比,脊髓损伤+电刺激组大鼠50%MWT和TWL均上升、TRPV1和CCL2转录和表达下降(P<0.05)、IL-6、IL-1β和TNF-α表达下降(P<0.05)。结论:脊髓电刺激可能通过抑制TRPV1和CCL2表达、减轻炎症反应,缓解脊髓损伤后神经病理性疼痛。Objective:To investigate the efficacy of spinal cord electrical stimulation on neuropathic pain in rats with spinal cord injury and its effects on the expression of transient receptor potential vanilloid 1(TRPV1)and C-C chemokine ligand 2(CCL2)in the spinal cord dorsal horn.Methods:Sixty-four female SD rats were randomly divided into four groups:Naïve group,sham surgery group,spinal cord injury group,and spinal cord injury+electrical stimulation group,with 16 rats in each group.Changes in paw mechanical withdrawal threshold(MWT)and thermal withdrawal latency(TWL)were observed before surgery,7 days post-surgery,and 14 days post-surgery.TRPV1,CCL2 mRNA and protein expression were detected with qPCR and Western Blot,respectively,and the levels of inflammatory cytokines IL-6,IL-1β,and TNF-αwere measured with ELISA.Results:Compared to the control and sham surgery groups,rats in the spinal cord injury group showed a decrease in 50%MWT and TWL at 7 and 14 days post-surgery,enhanced transcription and expression of TRPV1 and CCL2(P<0.05),and increased expression of IL-6,IL-1β,and TNF-α(P<0.001).Comparison with the spinal cord injury group,the spinal cord injury+electrical stimulation group exhibited an increase in 50%MWT and TWL,a reduction in transcription and expression of TRPV1 and CCL2(P<0.05),and a decrease in the expression of IL-6,IL-1β,and TNF-α(P<0.05).Conclusion:Spinal cord electrical stimulation may alleviate neuropathic pain after spinal cord injury by inhibiting the expression of TRPV1 and CCL2 and mitigating the inflammatory response.

关 键 词：神经病理性疼痛 脊髓电刺激 脊髓损伤 瞬时感受器电位香草酸受体1 C-C趋化因子配体2 

分 类 号：R651.2[医药卫生—外科学]