单宁酸对红色和绿色豌豆蚜酶活性的影响  

Effects of tannic acid on the enzymatic activity of red and green pea aphids(Acyrthosiphon pisum)

作　　者：梁瑶 吴宜亭 马瑞 魏江文 姚路 刘磊 宋丽雯 王森山 LIANG Yao;WU Yi-ting;MA Rui;WEI Jiang-wen;YAO Lu;LIU Lei;SONG i-wen;WANG Sen-shan(College of Plant Protection,Gansu Agricultural University,Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province,Lanzhou,Gansu Province 730070,China)

机构地区：[1]甘肃农业大学植物保护学院,甘肃省农作物病虫害生物防治工程实验室,甘肃兰州730070

出　　处：《草原与草坪》2024年第3期217-223,共7页Grassland and Turf

基　　金：甘肃省自然科学基金项目(20JR5RA028);甘肃省高等学校创新基金项目(2022A-060);甘肃农业大学公招博士科研启动项目(GAN-KYQD-2019-28)。

摘　　要：【目的】明确单宁酸对红色和绿色豌豆蚜酶活性的影响。【方法】设不同浓度单宁酸(1 mg/L、3 mg/L、5 mg/L)分别饲喂两种色型的5 d龄豌豆蚜,处理不同时间后测定豌豆蚜体内超氧化物歧化酶(SOD)、过氧化物酶(POD)、羧酸酯酶(CarE)及细胞色素P450s酶(CYP450)活性。【结果】豌豆蚜取食不同浓度单宁酸后,体内4种酶的响应程度不同。红色型豌豆蚜CarE与CYP450酶活性均在24 h处理的单宁酸浓度为3 mg/L时达最大值,分别为(0.029±0.002)μmol/(μg·min)和(0.006±0.0009)μmol/(μg·min),和对照间差异显著(P<0.05);绿色型豌豆蚜SOD与POD酶活性均在72 h处理的单宁酸浓度为3 mg/L时最高,分别达(0.028±0.004)μg/mg和(1.679±0.172)μg/(mg·min),和单宁酸浓度为5 mg/L的处理间差异显著(P<0.05)。【结论】红色和绿色豌豆蚜取食不同浓度单宁酸后,体内SOD、POD、CarE和CYP450酶活性均有不同程度的影响;相同处理时间,同种酶的活性在红绿色型豌豆蚜内的升高或降低程度不同;同种色型豌豆蚜取食不同浓度单宁酸后,体内不同酶的活性变化不同。【Objective】In order to determine the effects of tannic acid on the enzymatic activity of red and green Acyrthosiphon pisum.【Method】In this study,different concentrations of tannic acid(1 mg/L,3 mg/L and 5 mg/L)were fed with two color types of 5 d-aged pea aphid.SOD,POD,CarE and P450s enzyme activities in pea aphid were measured following different treatment times.【Result】The four enzymes responded differently to the different concentrations of tannic acid.With 24 h treatment,the maximum enzyme activities of CarE enzyme and P450 were 0.029±0.002μmol/(μg·min)and 0.006±0.0009μmol/(μg·min),respectively,which were significantly different(P<0.05),when tannic acid concentration was 3 mg/L.The enzyme activities of SOD and POD of green A.pisum were the highest and reached 0.028±0.004μg/mg and 1.679±0.172μg/(mg·min),respectively,when tannic acid concentration was 3 mg/L at 72 h.These were significantly different from tannic acid concentration of 5 mg/L treat-ment(P<0.05).【Conclusion】The different concentrations of tannic acid affected the activities of SOD,POD,CarE and P450 in red and green A.pisum.With the same treatment time,the activity of the same enzyme increased or de-creased to different degrees in the red and green type A.pisum.The activities of different enzymes in different concen-trations of tannic acid were different in the same type of A.pisum.

关 键 词：豌豆蚜 超氧化物歧化酶 过氧化物酶 羧酸酯酶 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治]