景宁木兰ZF-HD家族全基因组鉴定及其表达模式分析  

Genome-wide Identification of ZF-HD Family and Its Expression Pattern Analysis in Magnolia sinostellata

作　　者：朱森宇 陈小珺 马晶晶 任明杰 王晶晶 吴超 田丰 刘志高[1] 樊民亮 申亚梅[1] ZHU Sen-Yu;CHEN Xiao-Jun;MA Jing-Jing;REN Ming-Jie;WANG Jing-Jing;WU Chao;TIAN Feng;LIU Zhi-Gao;FAN Min-Liang;SHEN Ya-Mei(Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants/School of Landscape Architecture,Zhejiang A&F University,Hangzhou 311300,China;Zhejiang Academy of Forestry,Hangzhou 310023,China)

机构地区：[1]浙江省园林植物种质创新与利用重点实验室/浙江农林大学风景园林与建筑学院,杭州311300 [2]浙江省林业科学研究院,杭州310023

出　　处：《农业生物技术学报》2024年第8期1809-1820,共12页Journal of Agricultural Biotechnology

基　　金：浙江省“十四五”育种专项花卉协作组项目(2021C02071-3)。

摘　　要：景宁木兰(Magnolia sinostellata)为浙江省特有极小种群,受到冠层遮荫胁迫影响,目前处于濒危状态。锌指同源结构域(zinc finger homeodomain,ZF-HD)是一类在植物发育及生物、非生物胁迫响应过程中发挥重要作用的转录因子。为了明确景宁木兰ZF-HD基因家族在弱光胁迫下的作用机制,本研究利用转录组数据对MsZF-HD家族成员进行鉴定及表达模式解析。基于景宁木兰叶片转录组数据和望春玉兰(M.biondii)基因组数据,本研究鉴定了12个MsZF-HD家族成员,均定位于细胞核,MsZF-HD2b也定位于叶绿体。系统发育分析表明,MsZF-HD家族分为4个亚族,所有成员均含有ZF-HD_dimer结构域,同一亚族成员之间的基序(motif)数量及其类型高度保守。GO分析表明,MsZF-HD家族成员广泛参与景宁木兰的生长发育。组织特异性表达分析表明,大部分MsZF-HD家族成员在景宁木兰叶芽和叶中具有较高表达量,其中MsZF-HD8在茎和叶中均高表达,而MsZF-HD13和MsZF-HD14在花和茎中高表达,说明其参与调控花发育和茎的伸长过程。此外,对遮荫胁迫下MsZF-HDs基因的表达模式进行分析,发现MsZF-HDs对弱光胁迫产生积极响应,其中MsZF-HD1a、MsZF-HD1b、MsZF-HD1c、MsZF-HD2a、MsZFHD2b以及MsZF-HD14在受到弱光胁迫后显著下调表达(P<0.05);MsZF-HD13在弱光处理21和28 d的基因表达显著上调(P<0.05);MsZF-HD11、MsZF-HD12和MsZF-HD8在弱光处理7 d的基因表达显著上调(P<0.05),之后则呈下调趋势。本研究对景宁木兰ZF-HD家族成员进行了鉴定,并发现其在叶片、茎等组织中对弱光胁迫存在显著表达响应;筛选到MsZF-HD13与MsZF-HD8可能是景宁木兰响应遮荫,协调生殖生长、茎伸长及叶片衰老等过程的关键基因。本研究为解析景宁木兰遮荫响应机制提供理论依据。Stress from canopy shade is currently endangering a very small population of the indigenous Magnolia sinostellata found in Zhejiang province.A group of transcription factors known as zinc finger homeodomain(ZF-HD)is crucial for the plant development as well as the response to biotic and abiotic stress.In order to gain a better understanding of the mechanism underlying the MsZF-HD gene family under low-light stress,this study aimed to identify the members of the ZF-HD gene family and investigate their expression patterns based on transcriptome data.Twelve members of the MsZF-HD family were found using transcriptome data from M.sinostellata leaves and genomic data from M.biondii.The 12 MsZF-HDs located in the cell nucleus,and MsZF-HD2b also located in chloroplast.The ZF-HD_dimer structural domain was present in all members of the MsZF-HD gene family,which was revealed by phylogenetic analysis to be separated into 4 subfamilies.Additionally,the number and types of motifs were substantially conserved among the members of the same subfamily.According to GO analysis,members of the MsZF-HD family played a significant role in the expansion and evolution of M.sinostellata.Tissue-specific expression study revealed that most MsZF-HDs genes were substantially expressed in M.sinostellata leaf buds and leaves.MsZF-HD8 was highly expressed in both stems and leaves,while MsZF-HD13 and MsZF-HD14 were highly expressed in both flowers and stems,indicating that they might be involved in stem elongation and the regulation of floral development.Additionally,it was discovered that MsZF-HDs all responded favorably to low-light stress after studying the expression pattern of the MsZF-HDs gene under shade stress.Under low-light stress,the expression of MsZF-HD1a,MsZF-HD2b,MsZF-HD1b,MsZF-HD1c,MsZF-HD2a and MsZF-HD14 decreased.MsZF-HD13 gene expression was significantly upregulated after 21 and 28 d of low-light treatment(P<0.05).MsZF-HD11,MsZF-HD12 and MsZF-HD8 showed significant upregulation of gene expression after 7 d of low-light treat

关 键 词：锌指同源结构域(ZF-HD)转录因子家族 景宁木兰 弱光 基因表达 

分 类 号：S685.15[农业科学—观赏园艺]