新疆牧区羊产气荚膜梭菌病分离及鉴定  

Isolation and Identification of Clostridium Perfringens in Sheep in Pastoral Areas of Xinjiang Uygur Autonomous Region

作　　者：丁剑 赵耀国 艾日登才次克 范冰洁 史慧君[1] 马伟 DING Jian;ZHAO Yaoguo;AIRIDENGCAICIKE;FAN Bingjie;SHI Huijun;MA Wei(College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052,China;Xinjiang Uygur Autonomous Region Animal Disease Prevention and Control Center,Urumqi 830000,China)

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830052 [2]新疆维吾尔自治区动物疾病预防控制中心,乌鲁木齐830011

出　　处：《草食家畜》2024年第4期25-30,共6页Grass-Feeding Livestock

基　　金：新疆维吾尔自治区自然科学基金项目“新疆牧区羊梭菌病流行病学调查及防治技术研究”(2021D01A134)。

摘　　要：【目的】旨在探究新疆牧区羊产气荚膜梭菌病流行情况。【方法】于2023年春、秋季期间对来自新疆不同地区牧区羊群中疑似梭菌感染死亡羊150余份病料进行病原菌分离鉴定。采用人工感染试验和细菌鉴定仪、基质辅助激光解吸电离飞行时间质谱(MALDI-TOFMS)分别对其进行致病性检测和细菌鉴定。【结果】共分离出羊产气荚膜梭菌病16株,动物试验显示,分离菌株可引起小鼠死亡,致死率达80%。【结论】羊产气荚膜梭菌病在新疆牧区时有发生,做好免疫接种及科学管理,对控制羊梭菌病行之有效。【Objective】The aim is to explore the prevalence of sheep Clostridium perfringens disease in the pastoral areas of Xinjiang.【Methods】More than 150 samples of dead sheep suspected of clostridium infection from the sheep herds in 6 prefecturelevel cities and states such as Yili Prefecture,Bozhou,and Bazhou in Xinjiang during the spring and autumn of 2023 were isolated and identified for pathogenic bacteria.The artificial infection test,bacterial identification instrument,and matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS)were used respectively to conduct pathogenicity testing and bacterial identification.【Results】A total of 16 strains of sheep Clostridium perfringens disease were isolated,and the animal experiment showed that the isolated strains could cause the death of mice,with a mortality rate reaching 80%.【Conclusion】Sheep clostridium perfringens disease occurs from time to time in the pastoral areas of Xinjiang.Doing a good job in immunization and scientific management is effective in controlling sheep clostridial disease.

关 键 词：新疆牧区 羊产气荚膜梭菌病 细菌分离 细菌鉴定 

分 类 号：S858.26[农业科学—临床兽医学]