CRISPR genotyping and regional distribution characteristics of Yersinia pestis in natural plague focus of Tibet Autonomous Region  

作　　者：杨晓艳 YANG Xiaoyan(PlaguePrev&ContrKey Lab,Qinghai Endem Dis Prev&Contr Instit,Xining 810021)

机构地区：[1]PlaguePrev&ContrKey Lab,Qinghai Endem Dis Prev&Contr Instit,Xining 810021

出　　处：《China Medical Abstracts(Internal Medicine)》2024年第2期88-88,共1页中国医学文摘（内科学分册（英文版）

摘　　要：Objective Tostudy the clustered regularly interspaced short palindromic repeats(CRISPR)genotype of Yersinia pestis and its regional distribution characteristics in natural plaguefocusofTibet Autonomous Region.Methods A total of 125 representative Yersinia pestis strains isolated from natural plague focus in Tibet Autonomous Region at different times,regions,hosts and vectors were selected as experimental strains,and the phenol chloroform mixed extraction method was used to extract Yersinia pestis DNA.Three pairs of CRISPR primers(for YPa,YPb,YPc locus)were used to amplify the DNA of the experimental strains,and the CRISPR genotype of Yersinia pestis was determined by sequencing.Results All 125 strains of Yersinia pestis had three CRISPR locus:YPa,YPb,and YPc.A total of 18 spacer were found,including 8 in YPa loci,6 in YPb loci.

关 键 词：CRISPR distribution characteristics 

分 类 号：R378.61[医药卫生—病原生物学]