水生植物红宫廷草的快速繁殖研究  

A study on rapid propagation of aquatic plantRotala rotundifolia ‘Gontin’ by tissue culture

作　　者：杨水兰 杨茗越 徐月瑶 于琨 韦浩 刘柏玲[1] YANG Shuilan;YANG Mingyue;XU Yueyao;YU Kun;WEI Hao;LIU Boling(School of Life Sciences,Qufu Normal University,273165,Qufu,Shandong;Wengong Middle School in Wuyuan County,333200,Shangrao,Jiangxi,PRC)

机构地区：[1]曲阜师范大学生命科学学院,山东省曲阜市273165 [2]婺源县文公中学,江西省上饶市333200

出　　处：《曲阜师范大学学报（自然科学版）》2024年第3期112-116,共5页Journal of Qufu Normal University(Natural Science)

基　　金：国家自然科学基金(31700624);林木遗传育种国家重点实验室(中国林业科学研究院)开放课题(TGB2021005).

摘　　要：红宫廷草(Rotala rotundifolia‘Gontin’)是一种具有较高观赏和生态价值的水生植物,但是传统的繁殖方式效率较低.为在短时间内获得大量的红宫廷草小苗,该实验选取其茎段为外植体,以MS(Murashige and Skoog)为基本培养基进行植物组织培养研究其繁殖方法.实验结果表明,MS+0.5 mg/L 6-BA(6-Benzylaminopurine,6-苄氨基嘌呤)为红宫廷草最适的生芽培养基,生芽数为21.1个,叶绿素含量为9.83 mg/L;在MS培养基中添加1 g/L活性炭(Activated carbon,AC)有利于红宫廷草生根,其诱导生根的最适培养基为MS+1 g/L AC+0.6 mg/L IBA(Indole-butyric acid,吲哚丁酸).红宫廷草组培苗的移栽成活率达100%.该研究建立了红宫廷草的高效离体繁殖体系,为其规模化生产和利用提供了技术保障.Rotala rotundifolia‘Gontin’is a kind of aquatic plants,which has high ornamental and ecological value,but the traditional propagation method is difficult to meet the market demand.In order to obtain a large number of R.rotundifolia‘Gontin’seedlings in a short period of time,the buds were selected as explants,the optimum propagation method of plant tissue culture was studied using MS(Murashige and Skoog)as basic medium.The results showed that MS with 0.5 mg/L 6-BA(6-Benzylaminopurine)was the optimal medium for bud proliferation,with 21.1 buds and 9.83 mg/L chlorophyll content.Adding 1 g/L Activated carbon(AC)in MS medium was beneficial to rooting,and the optimum medium for rooting was MS with 1 g/L AC and 0.6 mg/L IBA(Indole-butyric acid).The transplant survival rate was 100%.In this research,a high frequency establishment of in vitro propagation system on R.rotundifolia‘Gontin’will provide a reliable technical support for its large scale production and utilization.

关 键 词：红宫廷草 组织培养 增殖 生根 

分 类 号：S681.9[农业科学—观赏园艺] Q813.12[农业科学—园艺学]