温振运气方对慢性疲劳综合征大鼠认知功能的影响  

Effects of Wenzhen Yunqi formula on cognitive function in rats with chronic fatigue syndrome

作　　者：李俊锋 张振贤[1] 史佳宁[1] 封鑫宇 LI Junfeng;ZHANG Zhenxian;SHI Jianing;FENG Xinyu(Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院,上海200437

出　　处：《吉林中医药》2024年第7期838-843,共6页Jilin Journal of Chinese Medicine

基　　金：国家自然科学基金项目(82274430);上海中医药大学附属岳阳中西医结合医院科研项目(2021yygm03)。

摘　　要：目的 探讨温振运气方对慢性疲劳综合征(chronic fatigue syndrome,CFS)大鼠认知功能的影响。方法 40只大鼠分为空白组、CFS模型组、CFS中药组(温振运气方组)、CFS西药组(盐酸多奈哌齐组),各10只,除空白组外,其余各组采用多重慢性应激方法建立慢性疲劳综合征模型。造模30天后,CFS中药组予温振运气方1.039 g/(kg·d)灌胃;CFS西药组予0.51 mg/(kg·d)盐酸多奈哌齐灌胃;空白组、CFS模型组予生理盐10 mL/(kg·d)灌胃,连续给药4周后进行行为学测试。行为学测试结束后采用ELISA法检测DA、ChAT、GABA水平,免疫组织化学染色法检测5-HT、Ng水平。结果 与模型组比较,CFS中药组与CFS西药组定位航行潜伏期、跳台错误次数、中央格停留时间均减少(P<0.01或P<0.05),跳台潜伏期、穿越平台次数、平台象限游泳时间、力竭游泳时间、悬尾挣扎次数均增加(P<0.01或P<0.05),CFS中药组水平运动时间、垂直运动时间和次数较模型组增加(P<0.01)、悬尾静止时间减少(P<0.01),CFS中药组和CFS西药组DA、5-HT、GABA、ChAT、Ng表达均水平升高(P<0.01或P<0.05)。结论 温振运气方可以调节CFS大鼠中枢神经递质、ChAT、Ng表达,减轻躯体疲劳,缓解负性情绪,改善认知功能障碍。Objective To investigate the effects of Wenzhen Yunqi formula(WZYQ formula) on cognitive function in rats with chronic fatigue syndrome(CFS) and its possible mechanism of action.Methods Forty rats were divided into 10 each in the blank group,CFS model group,WZYQ formula group,and donepezil hydrochloride group,and a CFS model was established using the multiple chronic stress method in rats of all the groups except the blank group.After 30 days of modeling,1.039g/(kg·d) of Wenzhen Yunqi formula was given by gavage in the WZYQ formula group,and 0.51mg/(kg·d) of donepezil hydrochloride was given by gavage in the western medicine group,while 10ml/kg of saline was given by gavage in the blank group and the model group,daily for 4 continuous weeks.After that,behavioral tests were performed in the four groups.The levels of DA,ChAT,and GABA were detected by the enzyme-linked immunosorbent assay(ELISA) and the levels of pentraxin(5-HT) and neurogranulin(Ng) were detected by immunohistochemical staining after the behavioral tests.Results Compared with the model group,the positioning navigation latency,number of platform jumping error,and central compartment dwell time were decreased in both the Chinese medicine and western medicine groups(P <0.01 or P <0.05),while the platform jumping latency,number of platform crossing,platform quadrant swimming time,exhaustion swimming time,and number of hanging tail struggle were all increased in both the Chinese medicine and western medicine groups(P<0.01 or P<0.05),and the horizontal movement time,vertical movement time and frequency were increased in the Chinese medicine group compared with the model group(P<0.01),while the hanging tail rest time was decreased(P<0.01).The expression levels of DA,5-HT,GABA,ChAT and Ng were all increased in both the Chinese medicine group and the western medicine group(P<0.01 or P <0.05).Conclusion Wenzhen Yunqi formula can regulate the expression of central neurotransmitters,ChAT,and Ng,reduce somatic fatigue,alleviate negative emotions,and improve c

关 键 词：慢性疲劳综合征 认知功能 温振运气方 神经递质 

分 类 号：R285.5[医药卫生—中药学]