反复输血铁过载大鼠心肝肾功能损伤的研究  

Study on the impairment of heart,liver and kidney function in rats with iron overload after repeated blood transfusion

作　　者：王华 邬云芬 沈丹萍 朱明辉 WANG Hua;WU Yunfen;SHEN Danping(Fenghua District Traditional Chinese Medicine Hospital of Ningbo City,Zhejiang 315500,China)

机构地区：[1]浙江省宁波市奉化区中医医院,宁波315500 [2]宁波大学附属第一医院

出　　处：《浙江创伤外科》2024年第7期1203-1208,共6页Zhejiang Journal of Traumatic Surgery

基　　金：浙江省宁波市奉化区科学技术局2021年度第六批科技计划项目(202106410)。

摘　　要：目的通过建立反复大量输血的Sprague Dawley远交群大鼠(SD大鼠)动物模型,探究反复输血导致的铁过载对心肝肾功能的损伤机制。方法建立反复大量输血的SD大鼠动物模型,记录体重变化和心肝肾组织质量变化,取实验动物血清检测其铁代谢和心肝肾功能指标,用Masson和普鲁士蓝染色分析心肝肾组织的损伤和铁沉积情况,Western blot实验检测心肝肾组织凋亡蛋白的表达情况,Q-PCR法检测心肝肾组织凋亡基因的表达水平。结果SD大鼠经尾静脉反复大量输血后,与对照组相比,其体重和心肝肾组织的质量均明显下降,铁离子水平明显升高,CK和CKMB等心功能指标明显升高,ALT、AST、LDH、TBIL和DBIL等肝功能指标均明显上升,组织病理学染色结果显示心肝肾组织纤维化和铁色素沉积明显,Western blot实验结果显示bax、cleaved caspase-3和p53等凋亡蛋白表达明显升高,Q-PCR实验结果显示bax、caspase-3和p53等凋亡基因表达明显上调,凋亡抑制基因bcl-2明显下调。结论SD大鼠经尾静脉反复大量输血后,体重和心肝肾组织的生长受抑制,血清生化相关指标改变,心肝肾功能受损,心肝肾组织纤维化和铁色素的沉积加剧,促进凋亡相关蛋白和基因的表达,抑制凋亡抑制基因的表达。Objective To explore the mechanism of iron overload induced by repeated blood transfusion on the function of heart,liver and kidney in SD rats.Methods The SD rat model of repeated massive blood transfusion was established,and the change of body weight and the difference of tissue mass of heart,liver and kidney were recorded,and the indexes of iron metabolism and heart,liver and kidney function in the serum of experimental animals were detected.The injury and iron deposition of heart,liver and kidney tissues were analyzed by masson and Prussian blue staining.The expression of apoptosis protein in heart,liver and kidney tissues were detected by Western blot,and the expression of apoptosis gene in heart,liver and kidney tissues were detected by Q-PCR.Results Compared with the control group,the weight and the weight of heart,liver and kidney of SD rats were significantly decreased,the level of iron ion was significantly increased,and the indexes of cardiac function such as CK and CKMB were significantly increased,the levels of Alt,AST,LDH,TBIL and DBIL were significantly increased.The histopathology staining showed that the fibrosis and iron pigment deposition in the heart,liver and kidney were obvious,the results of Western blot showed that the expression of BAX,cleaved Caspase-3 and p53 were significantly up-regulated.The results of Q-PCR showed that the expression of BAX,Caspase-3 and p53 was up-regulated,bCL-2(an inhibitor of apoptosis gene)was down-regulated.Conclusion After repeated massive blood transfusion via caudal vein in SD rats,body weight and the growth of heart,liver and kidney tissues were inhibited,serum biochemical indexes were changed,the function of heart,liver and kidney was damaged,the fibrosis of heart,liver and kidney tissues and the deposition of iron pigment were aggravated,promote the expression of apoptosis-related proteins and genes,inhibit the expression of apoptosis-inhibiting genes.

关 键 词：反复输血 铁过载 心肝肾损伤 凋亡蛋白 

分 类 号：R457.1[医药卫生—治疗学]