舒芬太尼调节TLR4/MyD88/NF-kB通路对重症肺炎大鼠肺损伤的影响  

Effect of Sufentanil on Lung Injury in Rats with Severe Pneumonia by Regulating TLR4/MyD88/NF-κB Pathway

作　　者：皇甫彪 张京硕[1] 叶海宾 李媛媛[1] HUANG Fubiao;ZHANG Jingshuo;YE Haibin(Tangshan Maternal and Child Health Hospital,Hebei Tangshan 063000,China)

机构地区：[1]河北省唐山市妇幼保健院麻醉科,河北唐山063000

出　　处：《河北医学》2024年第7期1082-1087,共6页Hebei Medicine

基　　金：河北省医学科学研究重点课题计划项目,(编号:20221749)。

摘　　要：目的:探讨舒芬太尼(Sufentanil,Sufen)对重症肺炎(Severe pneumonia,SP)大鼠肺损伤及Toll样受体4/髓样分化因子88/核因子-κB(Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-κB,TLR4/MyD88/NF-κB)通路的影响。方法:构建SP大鼠模型;将造模成功大鼠分为重症肺炎组(SP组)、舒芬太尼低、高剂量组(Sufen-L、Sufen-H组)、舒芬太尼高剂量+TLR4激活剂LPS组(Sufen-H+LPS组),每组24只,另取24只正常大鼠作为对照组(Control组);检测肺泡灌洗液中炎症因子水平及肺泡灌洗液沉淀物中炎症细胞数目;检测肺组织湿干重比(Wet/Dry,W/D);HE染色观察各组大鼠肺组织病理变化;Western blot检测肺组织中TLR4、MyD88、p-NF-κB p65/NF-κB p65表达水平。结果:SP组较Control组肺组织遭到破坏损伤严重,其中肺间质增厚,肺泡水肿并伴有大量炎性细胞浸润,TNF-α、IL-1β、IL-6水平和EOS、NEU炎症细胞数目及W/D比、TLR4、MyD88、p-NF-κB p65/NF-κB p65蛋白表达显著升高(P<0.05);Sufen-L、Sufen-H组较SP组大鼠肺组织损伤减轻,肺泡结构逐渐完整,水肿减轻,炎症细胞浸润逐渐减少,TNF-α、IL-1β、IL-6水平和EOS、NEU炎症细胞数目及W/D比、TLR4、MyD88、p-NF-κB p65/NF-κB p65蛋白表达显著降低,其中Sufen-H组优于Sufen-L组(P<0.05);Sufen-H+LPS组较Sufen-H组大鼠肺组织损伤加剧,肺泡结构破坏严重,肺泡水肿明显,炎性细胞浸润增加,TNF-α、IL-1β、IL-6水平和EOS、NEU炎症细胞数目及W/D比、TLR4、MyD88、p-NF-κB p65/NF-κB p65蛋白表达显著升高(P<0.05)。结论:Sufen改善SP大鼠的肺损伤,与抑制TLR4/MyD88/NF-κB信号通路有关。Objective:To investigate the effects of sufentanil(Sufen)on lung injury in rats with severe pneumonia(SP)and the Toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-κB(TLR4/MyD88/NF-κB)pathway.Methods:An SP rat model was constructed;the successfully modeled rats were grouped into severe pneumonia group(SP group),low-dose and high-dose sufentanil groups(Sufen-L,Sufen-H groups),and high-dose sufentanil+TLR4 activator LPS group(Sufen-H+LPS group),with 24 rats in each group.An additional 24 normal rats were used as the Control group.The levels of inflammatory factors in alveolar lavage fluid and the number of inflammatory cells in alveolar lavage fluid sediment were detected.The wet to dry weight ratio(W/D)of lung tissue was measured.HE staining was applied to observe the pathological changes in lung tissue of rats in each group.Western blot was applied to detect the expression levels of TLR4,MyD88,p-NF-κB p65/NF-κB p65 in lung tissue.Results:The lung tissue in the SP group was more severely damaged than that in the Control group,with thickening of the lung interstitium,alveolar edema,and extensive infiltration of inflammatory cells,the levels of TNF-α,IL-1β,IL-6,numbers of EOS and NEU inflammatory cells,W/D ratio,the protein expression of TLR4,MyD88,and p-NF-κB p65/NF-κB p65 were greatly increased(P<0.05).Compared with the SP group,the Sufen-L and Sufen-H groups showed reduced lung tissue damage,gradually intact alveolar structure,reduced edema,and reduced infiltration of inflammatory cells,the levels of TNF-α,IL-1β,IL-6,numbers of EOS and NEU inflammatory cells,W/D ratio,the protein expression of TLR4,MyD88,and p-NF-κB p65/NF-κB p65 were greatly reduced,and the Sufen-H group was superior to the Sufen-L group(P<0.05).Compared with the Sufen-H group,the Sufen-H+LPS group had more severe lung tissue damage,severe alveolar structural damage,great alveolar edema,and increased inflammatory cell infiltration in rats,the levels of TNF-α,IL-1β,IL-6,numbers of EOS and NEU inflammatory cells,W/

关 键 词：舒芬太尼 Toll样受体4/髓样分化因子88/核因子-κB通路 重症肺炎 肺损伤 

分 类 号：R563.1[医药卫生—呼吸系统]