脐血单个核细胞输注治疗大鼠宫腔粘连效果研究  

Therapeutic effect of umbilical cord blood mononuclear cells infusion on intrauterine adhesion in rats

作　　者：罗绮薇 史聪颖 马天宝 魏伟 Luo Qiwei;Shi Congying;Ma Tianbao;Wei Wei(Department of Obstetrics,Hexian Memorial Hospital,Panyu District,Guangzhou Guangdong 511400;Guangdong Cord Blood Bank,Guangdong Women's and Children's Hospital,Guangzhou Guangdong 510663,P.R.China)

机构地区：[1]广州市番禺区何贤纪念医院产科,广东广州511400 [2]广东省妇幼保健院广东省脐带血造血干细胞库,广东广州510663

出　　处：《中国计划生育和妇产科》2024年第7期57-61,71,F0003,共7页Chinese Journal of Family Planning & Gynecotokology

基　　金：番禺区科技计划项目基金资助项目(项目编号:2020-Z04-008);广州市基础研究计划基础与应用基础研究项目基金资助项目(项目编号:202201011771)

摘　　要：目的研究脐血单个核细胞(umbilical cord blood mononuclear cells,UCBMNCs)输注对大鼠宫腔粘连(intrauterine adhesions,IUA)的治疗效果及其作用机理。方法选取18只SPF级SD雌性大鼠随机分成对照组、模型组和UCBMNCs组。对照组大鼠开腹后即缝合关闭,模型组和UCBMNCs组利用开腹刮宫法构建大鼠宫腔粘连模型。模型组IUA大鼠进行尾静脉输注生理盐水;UCBMNCs组IUA大鼠进行尾静脉输注UCBMNCs。治疗2周后,收集各组大鼠的双侧子宫组织,检测大鼠子宫内膜腺体数量、纤维化面积占比,和TGF-β1、MMP-9、CTGF蛋白及mRNA水平的相对表达量。结果UCBMNCs组子宫形态比模型组有明显改善,内膜层腺体数目有所增多,子宫内膜纤维化百分比对照组为(20.61±8.56)%,模型组为(54.77±10.54)%,UCBMNCs组为(35.02±14.07)%,内膜层纤维化程度显著降低(P<0.05),局部子宫肌层可见胶原纤维细胞浸润,但程度比模型组低。模型组和UCBMNCs组的TGF-β1相对表达水平分别为(1.34±0.22)和(0.76±0.22),差异有统计学意义(t=3.228,P<0.05);UCBMNCs组TGF-β1的mRNA相对表达水平为(2.33±0.90),显著低于模型组(3.55±1.29)(P<0.05)。结论人源UCBMNCs可降低大鼠IUA模型的TGF-β1蛋白相对表达量水平,降低TGF-β1的mRNA相对表达水平,抑制宫腔内膜纤维化,改善宫腔粘连水平。Objective To investigate the therapeutic effect and mechanism of umbilical cord blood mononuclear cells(UCBMNCs)infusion on intrauterine adhesions(IUA)in rats.Methods 18 female SPF SD rats were randomly divided into control group,model group and UCBMNCs group.The rats in the control group were sutured immediately after laparotomy,and the rat model of intrauterine adhesions in the model group and UCBMNCs group were constructed by laparotomy curettage method.The model group was IUA model+intravenous infusion of normal saline.The UCBMNCs group was treated with intravenous infusion of UCBMNCs in the rat model of IUA.After 2 weeks of treatment,bilateral uterine tissues of each group of rats were collected to detect the number of endometrial glands,the ratio of fibrosis area,the protein and mRNA expression level of TGF-β1,MMP-9 and CTGF in endometrial tissues.Results Compared with the model group,the UCBMNCs group had a significant improvement in uterine morphology and increased endometrial glands.The percentage of endometrial fibrosis in the control group was(20.61±8.56)%,that in the model group was(54.77±10.54)%,and that in the UCBMNCs group was(35.02±14.07)%.The endometrial fibrosis of UCBMNCs group was significantly reduced(P<0.05),collagen fibrocyte infiltration could be seen in the local myometrium,but less than that in the model group.The relative expression levels of TGF-β1 in the model group and the UCBMNC group were(1.34±0.22)and(0.76±0.22),respectively,and the difference was statistically significant(t=3.228,P<0.05).The relative expression level of TGF-β1 mRNA in UCBMNCs group was(2.33±0.90),which was significantly lower than(3.55±1.29)in model group(P<0.05).Conclusions Human UCBMNCs can reduce the levels of TGF-β1 in the IUA model of rats,reduce the relative expression of TGF-β1 mRNA,inhibit endometrial fibrosis,and improve the IUA.

关 键 词：脐血单个核细胞 宫腔粘连 腺体数量 纤维化面积占比 TGF-Β1 

分 类 号：R711.4[医药卫生—妇产科学] R361[医药卫生—临床医学]