ACBP4-WRKY70-RAP2.12 module positively regulates submergence-induced hypoxia response in Arabidopsis thaliana  

作　　者：Mengyun Guo Yingjun Yao Kangqun Yin† Luna Tan Meng Liu Jing Hou Han Zhang Ruyun Liang Xinran Zhang Heng Yang Xiaoxiao Chen Jinrui Tan Yan Song Shangling Lou Liyang Chen Xuejing Liu Si Tang Yongqi Hu Jin Yan Wensen Fu Kai Yang Ruijia Zhang Xuerui Li Yao Liu Zhen Yan Wei Liu Yu Han Jianquan Liu Kangshan Mao Huanhuan Liu 

机构地区：[1]Key Laboratory for Bio‐resources and Eco‐environment&State Key Lab of Hydraulics&Mountain River Engineering,Sichuan Zoige Alpine Wetland Ecosystem National Observation and Research Station,Key Laboratory for Bio‐resource and Eco‐environment of Ministry of Education,College of Life Sciences,Sichuan University,Chengdu 610065,China

出　　处：《Journal of Integrative Plant Biology》2024年第6期1052-1067,共16页植物学报（英文版）

基　　金：the Natural Science Foundation of China(No.32270302 and No.32030006);the Fundamental Research Funds for the Central Universities(SCU2022D003)。

摘　　要：ACYL-CoA-BINDING PROTEINs(ACBPs)play crucial regulatory roles during plant response to hypoxia,but their molecular mechanisms remain poorly understood.Our study reveals that ACBP4 serves as a positive regulator of the plant hypoxia response by interacting with WRKY70,influencing its nucleocytoplasmic shuttling in Arabidopsis thaliana.Furthermore,we demonstrate the direct binding of WRKY70 to the ACBP4 promoter,resulting in its upregulation and suggesting a positive feedback loop.Additionally,we pinpointed a phosphorylation site at Ser638 of ACBP4,which enhances submergence tolerance,potentially by facilitating WRKY70's nuclear shuttling.Surprisingly,a natural variation in this phosphorylation site of ACBP4 allowed A.thaliana to adapt to humid conditions during its historical demographic expansion.We further observed that both phosphorylated ACBP4 and oleoyl-Co A can impede the interaction between ACBP4 and WRKY70,thus promoting WRKY70's nuclear translocation.Finally,we found that the overexpression of orthologous Bna C5.ACBP4and Bna A7.WRKY70 in Brassica napus increases submergence tolerance,indicating their functional similarity across genera.In summary,our research not only sheds light on the functional significance of the ACBP4 gene in hypoxia response,but also underscores its potential utility in breeding flooding-tolerant oilseed rape varieties.

关 键 词：ACBP4 Arabidopsis thaliana humid adaptation natural variation RAP2.12 WRKY70 

分 类 号：Q943.2[生物学—植物学]