Chromosomal-level genome and metabolome analyses of highly heterozygous allohexaploid Dendrocalamus brandisii elucidate shoot quality and developmental characteristics  

作　　者：Jutang Jiang Zeyu Zhang Yucong Bai Xiaojing Wang Yuping Dou Ruiman Geng Chongyang Wu Hangxiao Zhang Cunfu Lu Lianfeng Gu Jian Gao 

机构地区：[1]Key Laboratory of National Forestry and Grassland Administration,Beijing for Bamboo&Rattan Science and Technology,Institute of Gene Science and Industrialization for Bamboo and Rattan Resources,International Center for Bamboo and Rattan,Beijing 100102,China [2]College of Forestry,Basic Forestry and Proteomics Research Center,Fujian Agriculture and Forestry University,Fuzhou 350002,China [3]School of Life Science,Peking University,Beijing 100871,China [4]College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,China

出　　处：《Journal of Integrative Plant Biology》2024年第6期1087-1105,共19页植物学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2021YFD2200505,2018YFD0600101);the National Natural Science Foundation of China(32071849)。

摘　　要：Dendrocalamus brandisii(Munro)Kurz is a sympodial bamboo species with inimitable taste and flavorful shoots.Its rapid growth and use as high-quality material make this bamboo species highly valued for both food processing and wood applications.However,genome information for D.brandisii is lacking,primarily due to its polyploidy and large genome size.Here,we assembled a high-quality genome for hexaploid D.brandisii,which comprises 70chromosomes with a total size of 2,756 Mb,using long-read Hi Fi sequencing.Furthermore,we accurately separated the genome into its three constituent subgenomes.We used Oxford Nanopore Technologies long reads to construct a transcriptomic dataset covering 15 tissues for gene annotation to complement our genome assembly,revealing differential gene expression and post-transcriptional regulation.By integrating metabolome analysis,we unveiled that well-balanced lignin formation,as well as abundant flavonoid and fructose contents,contribute to the superior quality of D.brandisii shoots.Integrating genomic,transcriptomic,and metabolomic datasets provided a solid foundation for enhancing bamboo shoot quality and developing efficient gene-editing techniques.This study should facilitate research on D.brandisii and enhance its use as a food source and wood material by providing crucial genomic resources.

关 键 词：bamboo shoots Dendrocalamus brandisii(Munro)Kurz flavonoid GENOME lignin 

分 类 号：S795[农业科学—林木遗传育种]