微生物基因检测技术在鄂尔多斯盆地神府南气田勘探中的应用  

Application of Microbial Gene Detection Technology in Exploration of Shenfu South Gas Field in Ordos Basin

作　　者：徐伟 孙强[1] 梁玉楠 罗楚平 孟帅 张勇 XU Wei;SUN Qiang;LIANG Yunan;LUO Chuping;MENG Shuai;ZHANG Yong(China United Coalbed Methane Corp.,Ltd,Beijing 100016;Insoil Energy Technologies Co.,Ltd,Beijing 102200)

机构地区：[1]中联煤层气有限责任公司,北京100016 [2]英索油能源科技(北京)有限责任公司,北京102200

出　　处：《中国煤层气》2024年第2期3-6,共4页China Coalbed Methane

基　　金：中海石油“十四五”重大科技项目“陆上非常规天然气勘探开发关键技术”(KJGG2021-1000)。

摘　　要：针对鄂尔多斯盆地构造复杂,储层分布差异大,气藏富集规律不明等问题,本文采用微生物基因检测技术的荧光定量PCR法测量研究区样品中油气指示基因的丰度,同时利用高通量基因测序法检测样品中的烃类微生物种群类型,分析认为,本区主要有东北部、西部、南部三个烃类异常带,展布特征与沉积认识吻合,且西部、南部有亲缘关系,东北部与二者无亲缘关系,可能与致密气、煤层气不同层系烃的逸散有关。实钻表明,本技术对于鄂尔多斯盆地致密气和煤层气混源区的含油气性评价方面具有较好的适用性,为油气有利区带筛选提供参考依据。Given the complex structure,large differences in reservoir distribution,and unclear gas reservoir enrichment patterns in the Ordos Basin,this article employs the fluorescence quantitative PCR method of microbial gene detection technology to measure the abundance of oil and gas indicator genes in the samples of the study area.Additionally,high-throughput gene sequencing is used to detect the types of hydrocarbon microbial populations in the samples.Analysis indicates three major hydrocarbon anomaly zones in the northeastern,western,and southern parts of this area.The distribution characteristics are consistent with sedimentary understanding,and there is a close relationship between the western and southern zones.The northeast zone is unrelated to the other two,possibly due to the escape of hydrocarbons from different layers of tight gas and coalbed methane.Actual drilling shows that this technology has good applicability in the evaluation of oil and gas in the mixed-source area of tight gas and coalbed methane in the Ordos Basin,providing a reference for the screening of favorable oil and gas zones.

关 键 词：鄂尔多斯盆地 黄土塬地貌区 微生物基因检测 荧光定量检测 高通量基因测序 

分 类 号：P618.13[天文地球—矿床学]