艾绒中酸性黄36快速检测方法研究  

Study on Rapid Detection Methods of Acid Yellow 36 in Moxibustion

作　　者：赵培敬 陈林 宋颖 李中娥 黄涵 ZHAO Peijing;CHEN Lin;SONG Ying;LI Zhong'e;HUANG Han(Nanyang Inspection Testing Center for Products,Nanyang 473061,China;Northeast Forestry University,Harbin 150040,China)

机构地区：[1]南阳市产品质量检验检测中心,河南南阳473061 [2]东北林业大学,哈尔滨150040

出　　处：《中国现代应用药学》2024年第11期1524-1528,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：河南省市场监督管理局科技计划项目(2023sj19)。

摘　　要：目的建立艾绒中非法添加色素酸性黄36的快速检测方法。方法利用酸性黄36为酸碱指示剂,在pH值1.2(红)~2.3(黄)内变色的原理,以15%硫酸溶液为显色剂,对艾绒乙醇提取液进行初筛,再采用HPLC对初筛可疑阳性样品进行验证,最后采用LC-MS进行确认,检验所建立的理化快检方法的准确性。结果应用建立的酸性黄36快检方法在67批艾绒样品中检出3批阳性样品,经HPLC和LC-MS确认后,结果一致。结论所建立的方法准确、快速、便捷,可用于艾绒中酸性黄36的快速检测。OBJECTIVE To establish a rapid method for determining of acid yellow 36 in moxibustion.METHODS Based on the principle of acid yellow 36 as an acid-base indicator and discoloration in the pH range of 1.2(red)to 2.3(yellow),15%sulfuric acid solution was used as the color developing agent to screen the ethanol extract of the sample,and then HPLC method was used to verify the suspicious positive samples in the initial screening,and finally LC-MS method was used to confirm the accuracy of the established rapid physicochemical detection method.RESULTS The established method was applied to 67 batches of samples,and 3 positive samples were detected.The results were consistent with those of HPLC and LC-MS.CONCLUSION The method is accurate and sensitive,which can be used for rapid detection of acid yellow 36 in moxibustion.

关 键 词：艾绒 酸性黄36 快检方法 HPLC-DAD LC-MS/MS 

分 类 号：R917[医药卫生—药物分析学]