高效液相色谱法同时测定肥料中两种抑制剂的含量及国际标准制定研究  

Simultaneous determination of two inhibitors in fertilizers by high performance liquid chromatography and development of related international standard

作　　者：王新慧 田烨炜 黄河清 颜坤 储德韧 WANG Xin-hui;TIAN Ye-wei;HUANG He-qing;YAN Kun;CHU De-ren(Shanghai Research Institute of Chemical Industry,Co.,Ltd.,Shanghai 200062;Shanghai Institute of Chemical Industry Testing Co.,Ltd.,Shanghai 200062)

机构地区：[1]上海化工研究院有限公司,上海200062 [2]上海化工院检测有限公司,上海200062

出　　处：《中国土壤与肥料》2024年第5期240-246,共7页Soil and Fertilizer Sciences in China

摘　　要：为解决氮肥增效助剂测定方法存在的问题并实现对新型肥料的有效管控,建立了对肥料中N-正丁基硫代磷酰三胺和双氰胺同时测定的高效液相色谱分析方法。检验结果表明,该方法在线性范围内2种抑制剂的浓度与对应色谱峰面积的线性关系良好,线性相关系数均>0.9999,回收率分别为91.1%~104.8%和90.9%~107.6%,6次平行测定的相对标准偏差分别为1.94%和0.84%。与此同时,通过对全球18家国际实验室间比对试验数据的统计分析,表明该方法在兼顾前处理简单、分离效果好、检测准确的同时,又具有适用性广、精密度高的优势,ISO/DIS 6650可作为新的国际标准推广使用。In order to solve the problem existing in the determination method of nitrification inhibitors and achieve effective management and control of new fertilizers,an analytical method for simultaneous determination of NBPT and DCD in fertilizers was established by high performance liquid chromatography.The results showed that the linear relationship between the mass concentration of the two inhibitors and the value of the corresponding chromatographic peak area was good in the linear range.The correlation coefficients were all greater than 0.9999.The recoveries were 91.1%-104.8%and 90.9%-107.6%,and the relative standard deviations of 6 parallel determinations were 1.94%and 0.84%,respectively.At the same time,through the statistical analysis of an international comparison test between 18 international laboratories world-widely,it indicated that the method not only had the advantages of simple pretreatment,good separation effect and accurate detection,but also had the advantages of wide applicability and high precision.New international standard of ISO/DIS 6650 was a good testing method which could be used world-widely.

关 键 词：高效液相色谱 N-正丁基硫代磷酰三胺 双氰胺 国际标准 

分 类 号：TQ440.72[化学工程—化学肥料工业] O657.72[理学—分析化学]