一种基于多路纳米孔测序技术的多种血清型沙门菌快速分型方法  

A rapid detection method for multiple serotypes of Salmonella based on multiplex nanopore sequencing

作　　者：王东帅 师丹阳 李君文 金敏 WANG Dongshuai;SHI Danyang;LI Junwen;JIN Min(Tianjin Key Laboratory of Risk Assessment and Control for Environment&Food Safety,Academy of Military Medical Sciences,Academy of Military Science,Tianjin 300050,China)

机构地区：[1]军事科学院军事医学研究院,天津市环境与食品安全风险监督技术重点实验室,天津300050

出　　处：《军事医学》2024年第5期389-394,共6页Military Medical Sciences

摘　　要：目的构建基于多路纳米孔测序技术的本地化沙门菌血清型分型方法,评估血清型数量和测序时间对分型结果的影响,为多种血清型沙门菌的同时快速分型提供方法。方法首先,利用纳米孔测序技术建立多菌株测序方法。其次,基于沙门菌核心基因组数据库构建本地化沙门菌血清型分型流程。最后评估血清型数量和测序时间对分型结果的影响。结果建立的多种血清型沙门菌分型方法准确率和特异性均可达到100%,且在2 h测序时间内可完成6株以上不同血清型的沙门菌快速准确分型。结论该方法能够同时对多株血清型沙门菌快速分型,有效提高沙门菌血清型的鉴定效率,为环境水体中沙门菌血清型的快速鉴定提供有力支持。Objective To develop a localized Salmonella serotyping method based on multiplex nanopore sequencing technology,and evaluate the impact of serotype quantity and sequencing time on the serotyping results,aiming to provide a method for simultaneous rapid serotyping of various Salmonella serotypes.Methods Initially,a multiplex sequencing method was established by using nanopore sequencing technology.Subsequently,a localized Salmonella serotyping workflow was developed based on the coregenome MLST database of Salmonella.Finally,the effects of serotype quantity and sequencing time on serotyping results were evaluated.Results The established method for serotyping various Salmonella serotypes achieved an accuracy and specificity of 100%,with the ability to accurately serotyping at least 6 Salmonella serotypes within 2 hours.Conclusion The multiplex nanopore sequencing method enables rapid serotyping of multiple Salmonella serotypes simultaneously,significantly improving the identification efficiency of Salmonella serotypes and providing robust support for the rapid serotyping of Salmonella serotypes in environmental water samples.

关 键 词：多路纳米孔测序 沙门菌 血清型 快速分型 

分 类 号：Q93‐332[生物学—微生物学] Q939.123