羟基红花黄色素A抑制血管紧张素Ⅱ介导的心脏成纤维细胞肌化  

Inhibition of angiotensinⅡ-mediated cardiac fibroblast myofibroblast transformation by hydroxy crocetin A

作　　者：蒋洁 王立群 刘雪茹 杨艳 谭晓秋 陈唐葶 JIANG Jie;WANG Liqun;LIU Xueru;YANG Yan;TAN Xiaoqiu;CHEN Tangting(Key Laboratory of Medical Electrophysiology of Ministry of Education,Medical Electrophysiology Key Laboratory of Sichuan Province,Institute of Cardiovascular Research,Southwest Medical University,Luzhou 646000,China;Basic Medicine Research Innovation Center for Cardiometabolic Diseases,Ministry of Education,Southwest Medical University,Luzhou 64600,China;Department of Anesthesiol-ogy,The Affiliated Hospital,Southwest Medical University,Luzhou 646000,China;School of Basic Medical Science,Southwest Medical University,Luzhou 646000,China)

机构地区：[1]西南医科大学医学电生理学教育部重点实验室,医学电生理四川省重点实验室,心血管医学研究所,泸州646000 [2]西南医科大学教育部代谢性心血管疾病医药基础研究创新中心,泸州646000 [3]西南医科大学附属医院麻醉科,泸州646000 [4]西南医科大学基础医学院,泸州646000

出　　处：《西南医科大学学报》2024年第4期306-310,共5页Journal of Southwest Medical University

基　　金：国家自然科学基金(81900277);四川省科技计划联合创新专项(2022YFS0607,2022YFS0632,2022YFS0630);泸州市科技计划项目(2023JYJ004)。

摘　　要：目的研究羟基红花黄色素A对血管紧张素Ⅱ介导的心脏成纤维细胞向肌成纤维细胞转化的影响。方法实验细胞随机分为正常对照组、Ang-Ⅱ组、Ang-Ⅱ+HSYA(5μM)组、Ang-Ⅱ+HSYA(25μM)组、Ang-Ⅱ+HSYA(50μM)组和Ang-Ⅱ+HSYA(100μM)组。使用划痕实验和Transwell小室侵袭实验检测细胞迁移侵袭情况,DCFH-DA荧光探针检测细胞内活性氧(reactive oxygenspecies,ROS)水平,Western blot检测α-SMA、CollagenⅠ、CollagenⅢ及转化生长因子β1(transforming growthfactor-β1,TGF-β1)的蛋白表达水平。结果Ang-Ⅱ促进心脏成纤维细胞迁移、增加ROS产生;而HSYA抑制了Ang-Ⅱ介导的心脏成纤维细胞迁移以及ROS产生;Western blot发现HSYA抑制了Ang-Ⅱ介导的心脏成纤维细胞α-SMA、CollagenⅠ、CollagenⅢ及TGF-β1的蛋白表达,差异具有统计学意义(P<0.05)。结论HSYA通过减少ROS的产生和下调TGF-β1信号通路抑制Ang-Ⅱ诱导心肌成纤维细胞向肌成纤维细胞分化。Objective To study the effects of Hydroxysafflor yellow A(HSYA)on angiotensinⅡ(Ang-Ⅱ)-mediated myocar-dial fibrosis.Methods HFC-aa cells were randomly divided into the control group,the Ang-Ⅱgroup,the Ang-Ⅱ+HSYA(5μM)group,the Ang-Ⅱ+HSYA(25μM)group,the Ang-Ⅱ+HSYA(50μM)group,and the Ang-Ⅱ+HSYA(100μM)group.Cells in the Ang-Ⅱgroup were treated with 0.5μM Ang-Ⅱfor 24 hours,and cells in the Ang-Ⅱ+HSYA groups were treated with HSYA(5,25,50,and 100μM)and Ang-Ⅱ(0.5μM)for 24 hours.Cells in the control group were treated with the same volume of PBS.Cell wound healing and transwell migration assays were used to determine the cell migration.DCFH-DA fluorescent probe was utilized to de-tect the intracellular ROS level,and western blot was used to evaluate the protein expression ofα-SMA,CollagenⅠ,CollagenⅢand TGF-β1.Results Ang-Ⅱcould increase the migration and ROS production of cardiac fibroblasts.HSYA inhibited Ang-Ⅱ-mediated cardiac fibroblasts migration and ROS production.Western blot showed that HSYA inhibited Ang-Ⅱ-mediated protein expression ofα-SMA,CollagenⅠ,CollagenⅢand TGF-β1 in cardiac fibroblasts,and the difference was statistically significant(P<0.05).Con-clusion HSYA inhibited the differentiation of cardiac fibroblasts into myofibroblasts induced by Ang-Ⅱby reducing the production of reactive oxygen species(ROS)and down-regulating the TGF-β1 signaling pathway.

关 键 词：心肌纤维化 羟基红花黄色素A 血管紧张素Ⅱ 活性氧 转化生长因子Β1 

分 类 号：R961[医药卫生—药理学]