拟南芥NTMC2T4的抗体制备及特异性检测  

Antibody Preparation and Specific Detection of NTMC2T4 in Arabidopsis

作　　者：赵媛 任杰 胡俊杰 Zhao Yuan;Ren Jie;Hu Junjie(College of Life Sciences,Nankai University,Tianjin 300071,China;Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China)

机构地区：[1]南开大学生命科学学院,天津300071 [2]中国科学院生物物理研究所,北京100101

出　　处：《南开大学学报（自然科学版）》2024年第3期30-34,共5页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金(31171284)。

摘　　要：拟南芥中的NTMC2T4是含N端跨膜区和C2结构域蛋白家族中的一员,有研究指出在逆境条件下,NTMC2T4缺失突变体比野生型更抗旱、更耐盐,但其具体功能机制还未有报道.为了深入研究NTMC2T4,课题组纯化了NTMC2T4胞浆段,并制备了相应的多克隆抗体.随后利用抗体进行免疫印迹和免疫共沉淀实验,发现NTMC2T4抗体可以检测到内源和外源NTMC2T4的表达.实验结果证明了NTMC2T4抗体的特异性,为后续将要开展的NTMC2T4功能机制以及与其他蛋白互作的研究奠定了重要基础.NTMC2T4 is a member of the N-terminal transmembrane region and C2 domain protein family in Arabidopsis.Studies have shown that NTMC2T4 defective mutants are more resistant to drought and salt than wild type under stress conditions,but the mechanism of function has not been reported.In order to further study NTMC2T4,the research team purified the NTMC2T4 cytoplasmic segment and prepared the corresponding polyclonal antibodies.Immunoblotting and co-immunoprecipitation experiments were performed using antibodies.It was found that NTMC2T4 antibody can detect the expression of endogenous and exogenous NTMC2T4.The experimental results proved the specificity of the NTMC2T4 antibody,which lays an important foundation for the future research on the functional mechanism of NTMC2T4 and its interaction with other proteins.

关 键 词：NTMC2T4 蛋白纯化 抗体制备 免疫印迹 

分 类 号：Q2[生物学—细胞生物学]