基于数据挖掘分析验证清代医家治疗糖尿病的用药规律和作用机制  

Base on Data Mining to Analyze and Verify the Rule and Mechanism of Physicians in the Qing Dynasty in Treating Diabetes

作　　者：王任 李怡梅 卢伟炽 王增勇 樊毓运[5,7] Wang Ren;Li Yimei;Lu Weichi;Wang Zengyong;Fan Yuyun(Guangzhou University of Chinese Medicine,Linyi People's Hospital Postgraduate Training Base,Linyi 276000,China;Guangzhou University of Chinese Medicine,Guangzhou 510000,China;The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,China;Guangdong Clinical Research Academy of Chinese Medicine,Guangzhou 510405,China;Linyi People's Hospital,Linyi 276000,China;Shandong Provincial Clinical Research Center for Geriatric Diseases,Linyi 276000,China;Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)

机构地区：[1]广州中医药大学临沂市人民医院研究生培养基地,山东临沂276000 [2]广州中医药大学,广东广州510000 [3]广州中医药大学第一附属医院,广东广州510405 [4]广东省中医临床研究院,广东广州510405 [5]临沂市人民医院,山东临沂276000 [6]山东省老年疾病临床医学研究中心,山东临沂276000 [7]中国中医科学院广安门医院,北京100053

出　　处：《南开大学学报（自然科学版）》2024年第3期58-64,共7页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金面上项目(82174284);山东省中医临床优秀人才研修项目(鲁卫函[2022]233号);临沂市重点研发计划(医学类)(2023YX0092,2023YX0104)。

摘　　要：应用中医传承辅助平台挖掘清代医家用药规律,发现药物组合和新处方,应用网络药理学技术对新处方进行药理学机制的分析,并用糖尿病大鼠模型进行验证.研究发现,清代医家用药以补虚药、收涩药为主.对新处方“双苓泽丹方”进行网络药理学分析,得出主要成分为kaempferol、catechin、quercetin等,关键靶点有AKT1、TP53、TNF、IL6、JUN、VEGFA、CASP3、IL1B等,主要涉及到IL-17、PI3K-Akt、Relaxin等通路.动物实验显示双苓泽丹方组血糖下降,进水量、排泄量下降,血清空腹胰岛素、胰岛素抵抗指数、糖化血红蛋白和TNF-α较模型组改善.ALT、AST、ALP和UREA较模型组较低,提示双苓泽丹方可能通过改善胰岛素抵抗调控糖尿病大鼠的血糖,且有一定的肝肾保护功能.Based on data mining analysis,this study verifies the rule and mechanism of physicians in the Qing Dynasty in treating diabetes.TCMIAP was used to explorer the rule of physicians in the Qing Dynasty,discover drug combinations and potential new prescriptions.Network pharmacology technologywas applied to analyze the mechanism of new prescriptions,which was verified with the model of diabetes rats.The research found that physicians in the Qing Dynasty often used tonic deficiency medicine and astringent medicine.A network pharmacology analysis was conducted on the new prescription"SLZDF",and the main components were identified as Kaempferol,catechin,quercetin,etc.Key targets include AKT1,TP53,TNF,IL6,JUN,VEGFA,CASP3,IL1B,etc.mainly pathways such as IL-17,PI3K Akt,and Relaxin.Animal experiments showed that in the treatment group,FBG,water intake,excretion,FINS,HOMAIR,HbA1c,and TNF-α,ALT,AST,ALP,UREA decreased.

关 键 词：清代 糖尿病 中医传承辅助平台 网络药理学 

分 类 号：R259[医药卫生—中西医结合]