利用秀丽隐杆线虫模型研究CT14在发育中的功能  

Study on the developmental function of CT14 using the model organism Caenorhabditis elegans

作　　者：杨舒雯 陈娟 杨琴 雷鸣 黄晨辉 YANG Shuwen;CHEN Juan;YANG Qin;LEI Ming;HUANG Chenhui(Shanghai Institute of Precision Medicine,Shanghai Ninth People′s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200125,China)

机构地区：[1]上海交通大学医学院附属第九人民医院上海精准医学研究院,上海200125

出　　处：《上海交通大学学报（医学版）》2024年第7期871-882,共12页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(31971137)。

摘　　要：目的·使用模式生物秀丽隐杆线虫(简称线虫)研究癌-睾丸抗原14(cancer-testis antigen 14,CT14)对其胚胎及幼虫发育的影响,探索CT14在发育中的潜在功能和作用机制。方法·利用显微注射构建可诱导表达人源CT14(HsCT14)、缺失CT14特异性中间序列(CT14-specific intermediate region,CIR)的截短突变体HsCT14_(ΔCIR)及绿色荧光蛋白对照的转基因线虫品系,观察并比较全长和截短突变体CT14表达后对线虫虫卵的胚胎发育及幼虫发育过程的影响。构建可诱导表达食蟹猕猴(Macaca fascicularis)和倭狐猴(Microcebus murinus)CT14的线虫品系,比较异源表达不同灵长类种属来源的CT14对线虫虫卵孵化率及幼虫成虫率的影响。通过Smart-seq转录组测序技术分析CT14表达引起的线虫胚胎的基因表达差异,并采用京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析和基因集富集分析(Gene Set Enrichment Analysis,GSEA),进一步探索线虫胚胎中受CT14影响的相关生物学过程及通路。结果·HsCT14及其截短突变体HsCT14_(ΔCIR)的诱导表达显著降低了线虫虫卵孵化率,其中HsCT14的表达对孵化率的影响较大。微分干涉对比(differential interference contrast,DIC)显微技术观察显示,表达HsCT14的线虫胚胎在发育的comma期表现出明显的形态学异常。HsCT14及其截短突变体HsCT14_(ΔCIR)表达后的线虫幼虫成虫率显著低于绿色荧光蛋白对照组,并出现生长阻滞现象,其中HsCT14的表达对成虫率的影响较大。食蟹猕猴CT14(MfCT14)的表达对线虫虫卵孵化率及幼虫成虫率的影响与人源HsCT14相似,而倭狐猴CT14(MmCT14)的表达对上述指标的影响显著低于HsCT14和MfCT14。Smart-seq转录组测序结果显示,CT14的表达可能影响线虫胚胎的多个生物学过程,涉及ATP依赖的染色质重塑过程和DNA复制通路等。结论·CT14的异源表达显著干扰线虫的胚胎发育和幼虫发育,CIR起了Objective·To investigate the effects of the cancer-testis antigen 14(CT14)on embryonic and larval development in nematodes by using the model organism Caenorhabditis elegans(C.elegans),aiming to uncover its potential functions and mechanisms during development.Methods·Transgenic C.elegans strains were constructed by using microinjection for the inducible expression of human CT14(HsCT14),a truncated mutant of CT14(HsCT14_(ΔCIR))lacking CT14-specific intermediate region(CIR),and a green fluorescent protein(GFP)control.The impacts of full-length and truncated mutant CT14 on nematode embryonic and larval development were analyzed and compared.Additionally,transgenic C.elegans strains with inducible expression of CT14 from various primates,including the crab-eating macaque(Macaca fascicularis)and mouse lemur(Microcebus murinus),were also constructed to assess the effects on egg hatching and larval-to-adult transformation rates.The differential gene expression in nematode embryos induced by CT14 was analyzed by Smart-seq transcriptome sequencing,with further insights gained through KEGG(Kyoto Encyclopedia of Genes and Genomes)and GSEA(Gene Set Enrichment Analysis),to explore the involved biological processes and pathways.Results·The induced expression of HsCT14 and its truncated mutant HsCT14_(ΔCIR) significantly reduced the hatching rate of nematode eggs,with a more pronounced effect observed in HsCT14-expressing strains.Differential interference contrast(DIC)microscopy imaging revealed significant morphological abnormalities in embryos expressing HsCT14 during the comma stage.Nematodes expressing HsCT14 or HsCT14_(ΔCIR) exhibited developmental arrest in larvae and substantially lower larval-to-adult transformation rates compared to the GFP control.The impact was more pronounced in nematodes expressing HsCT14 than those with HsCT14_(ΔCIR).The expression of Macaca fascicularis CT14(MfCT14)exhibited significant effects on the hatching rate and adult transformation rate,similar to that of HsCT14,while the expressi

关 键 词：癌-睾丸抗原 癌-睾丸抗原14 秀丽隐杆线虫 胚胎发育 幼虫发育 

分 类 号：R714.51[医药卫生—妇产科学]