亚抑菌浓度美罗培南对多重耐药鲍曼不动杆菌生物膜作用的研究  

Study on the Effect of Meropenem at Subinhibitory Concentration for the Biofilmof Multi-drug Resistant Acinetobacter Baumannii

作　　者：罗艳[1] 宋沧桑[1,2] 王波[1] 任宝军[2] 李兴德[1,2] 张思敏[3] 方甜甜 LUO Yan;SONG Cang-sang;WANG Bo;REN Bao-jun;LI Xing-de;ZHANG Si-min;FANG Tian-tian(Affiliated Ganmei Hospital of Kunming Medical University,Yunnan Kunming 650000,China;The First People′s Hostpital of Kunming,Yunnan Kunming 650000,China;Kunming University,Yunnan Kunming 650214,China)

机构地区：[1]昆明医科大学附属甘美医院,云南昆明650000 [2]昆明市第一人民医院,云南昆明650000 [3]昆明学院,云南昆明650214

出　　处：《中国药物评价》2024年第3期217-221,共5页Chinese Journal of Drug Evaluation

基　　金：昆明市科技局项目(No.2019KJJH073);昆明市卫生科技人才培养项目[项2020-SW(后备)-36]。

摘　　要：目的:探讨亚抑菌浓度美罗培南对多重耐药鲍曼不动杆菌(MDR-Ab)生物膜作用的影响。方法:采用微量肉汤稀释法测定某三甲医院从临床血液标本中分离的6株碳青霉烯类耐药的多重耐药鲍曼不动杆菌(MDR-AB)对美罗培南的最低抑菌浓度(MIC),结晶紫半定量法测定菌株生物膜形成能力。A组实验用96孔聚苯乙烯板体外构建MDR-AB生物膜的模型,用亚抑菌浓度美罗培南作用于成熟生物膜;B组实验用96孔聚苯乙烯板将MDR-AB浮游菌液与亚抑菌浓度美罗培南共培养。通过结晶紫半定量染色法对生物膜进行染色,再用多功能酶标仪测生物膜在560 nm波长处的OD值。结果:A组a、b、c中所形成生物膜OD值与对照组d组比较,48 h结果有统计学意义(P<0.05),而96h无统计学意义(P>0.05);B组X、Y、Z的生物膜形成过程中,48 h和96 h后OD值与对照组W有统计学意义(P<0.05)。结论:MDR-Ab有形成生物膜的能力;亚抑菌浓度美罗培南具有破坏成熟生物膜的能力,且其破坏能力随着浓度的升高而增强;1/2 MIC、MIC浓度的美罗培南可能抑制MDR-Ab生物膜形成,而1/4 MIC浓度的美罗培南则可能诱导MDR-Ab生物膜形成。Objective:To investigate the effect of sub-inhibitory concentrations of meropenem on the biofilm action of multi-drug resistant Acinetobacter baumannii(MDR-Ab).Methods:The lowest inhibitory concentration(MIC)of meropenem was measured by broth microdilution.Group A:The biofilm model was constructed with 96-well plate,The mature biofilm was destroyed with meropenem of 1/4 MIC,1/2 MIC and MIC in experimental groups a,b and c,respectively,Group d control group cultured without meropenem for 48 h,96 h,Analysis of bacterial biofilms by crystal violet staining,The OD value of the bacterial biofilm at the wavelength of 560 nm was then measured by a multi-function microplate reader.Group B:The bacterial solution was incubated together with meropenem,Meropenem was added 1/4 MIC,1/2 MIC,MIC to the sub-inhibitory concentration in experimental groups X,Y and Z and cultured MDR-Ab,Group W control group was cultured without meropenem for 48h,96h,Biofilms were determined by the same method.Results:The OD values of the biofilm in group a,b and c in group A and the OD values in the control group d and the OD values in significant difference(P<0.05),but no significant difference in 96 h(P>0.05).The OD values in group X,Y,Z and the OD value of control W in 48 h and 96 h in group B(P<0.05).Conclusion:MDR-Ab has biofilm formation.Group A:meropenem is able to destroy mature biofilm and increases with concentration.Group B:MDR-Ab biofilm formation at MIC and 1/2 MIC,may induce biofilm formation at 1/4 MIC.

关 键 词：多重耐药鲍曼不动杆菌(MDR-Ab) 生物膜 亚抑菌浓度 美罗培南 

分 类 号：R962.2[医药卫生—药理学]