不同根瘤菌菌株对Cu和Cd污染土壤中苜蓿的影响  

Effects of different rhizobial strains on Medicago sativa in Cu-and Cd-contaminated soil

作　　者：郝思迪 王振 董官勇 万益帆 邓静 胥思印 田鸿 余秀梅 HAO Sidi;WANG Zhen;DONG Guanyong;WAN Yifan;DENG Jing;XU Siyin;TIAN Hong;YU Xiumei(College of Resources,Sichuan Agricultural University,Chengdu 611130,China;Cangxi Kiwifruit Industry Development Bureau,Guangyuan 628499,China)

机构地区：[1]四川农业大学资源学院,四川成都611130 [2]苍溪县猕猴桃产业发展局,四川广元628499

出　　处：《植物资源与环境学报》2024年第4期30-38,49,共10页Journal of Plant Resources and Environment

基　　金：四川省科技成果转移转化示范项目(2022ZHCG0030);成都市科技项目(2023-YF08-00004-SN);德阳市重点研发项目(2022NZ015)。

摘　　要：为了探明不同根瘤菌菌株对Cu和Cd污染土壤中苜蓿(Medicago sativa Linn.)的影响,对Cu和Cd污染土壤中(质量分数分别为20和2 mg·kg^(-1))接种根瘤菌菌株SM14-3、SM3-2、SM1和SM13-2后苜蓿的生长指标、主要营养元素含量和相应重金属含量进行了比较,并对这些指标进行了Pearson相关性分析。结果表明:Cu和Cd污染土壤中,未接种根瘤菌但施氮(阳性对照)的苜蓿地上和地下部Cu和Cd含量均显著(P<0.05)低于未接种根瘤菌且不施氮(阴性对照),生长指标及地上和地下部N和P含量多显著高于阴性对照,而地上和地下部K含量多低于阴性对照。Cu污染土壤中,接种SM14-3、SM3-2和SM1的苜蓿地上和地下部Cu含量均显著低于阴性对照,接种4个根瘤菌菌株的苜蓿株高、根长、单株地上和地下部干质量、地上和地下部N和P含量以及地下部K含量多显著高于阴性对照。Cd污染土壤中,接种SM14-3的苜蓿地上和地下部Cd含量以及接种SM3-2和SM1的苜蓿地上部Cd含量均显著低于阴性对照;接种4个根瘤菌菌株的苜蓿单株地上和地下部干质量多显著高于阴性对照;接种SM14-3的苜蓿地上和地下部N、P、K含量均显著低于阴性对照,而接种SM3-2、SM1和SM13-2的苜蓿地上和地下部N、P、K含量变化各异。比较来看,Cu和Cd污染土壤中,接种SM14-3和SM3-2的苜蓿各指标总体上表现较好。相关性分析结果表明:地上部指标中,Cu含量与N含量、P含量和株高分别呈极显著(P<0.01)负相关、显著正相关和显著负相关,Cd含量与株高呈显著负相关;地下部指标中,Cd含量与单株地下部干质量和根长呈极显著负相关。研究结果显示:供试根瘤菌菌株对Cu和Cd污染土壤中苜蓿的生长总体上有促进作用,并在一定程度上阻隔苜蓿对土壤中Cu和Cd的吸收,其中,SM14-3和SM3-2的作用突出,可作为Cu和Cd污染土壤中苜蓿高效、安全生产的备选菌株。In order to investigate the effects of different rhizobial strains on Medicago sativa Linn.in Cu-and Cd-contaminated soil,the growth indexes,major nutrient element contents,and corresponding heavy metal contents of M.sativa inoculated with rhizobial strains SM14-3,SM3-2,SM1,and SM13-2 in Cu-and Cd-contaminated soil(mass fractions of 20 and 2 mg·kg^(-1)respectively)were compared,and the Pearson correlation analysis was conducted for these indexes.The results show that in Cu-and Cd-contaminated soil,the Cu and Cd contents in above-and under-ground parts of M.sativa uninoculated with rhizobia but applied with nitrogen(positive control)are both significantly(P<0.05)lower than those of M.sativa uninoculated with rhizobia and not applied with nitrogen(negative control),the growth indexes and the N and P contents in above-and under-ground parts are mostly significantly higher than those of the negative control,while the K contents in above-and under-ground parts are mostly lower than those of the negative control.In Cu-contaminated soil,the Cu contents in above-and under-ground parts of M.sativa inoculated with SM14-3,SM3-2,and SM1 are all significantly lower than those of the negative control,and the plant height,root length,dry masses of above-and under-ground parts per plant,N and P contents in above-and under-ground parts,and K content in under-ground part of M.sativa inoculated with four rhizobial strains are mostly significantly higher than those of the negative control.In Cd-contaminated soil,the Cd contents in above-and under-ground parts of M.sativa inoculated with SM14-3,as well as the Cd content in above-ground part of M.sativa inoculated with SM3-2 and SM1,are all significantly lower than those of the negative control;the dry masses of above-and under-ground parts per plant of M.sativa inoculated with four rhizobial strains are mostly significantly higher than those of the negative control;the N,P,and K contents in above-and under-ground parts of M.sativa inoculated with SM14-3 are significantly lower than

关 键 词：苜蓿 根瘤菌 重金属污染土壤 相关性分析 共生固氮 

分 类 号：Q948.1[生物学—植物学] S551.7[农业科学—作物学]