桑辛素对胶质瘤细胞株U87迁移与侵袭的影响  

Effects of morusin on the migration and invasion of U87 cells

作　　者：唐冬 高文宏[1] 张华平[1] 陈谦学[2] TANG Dong;GAO Wenhong;ZHANG Huaping;CHEN Qianxue(Department of Neurosurgery,Jingzhou Hospital Affiliated to Yangtze University,Jingzhou 434020,China;Department of Neurosurgery,Renmin Hospital of Wuhan University)

机构地区：[1]长江大学附属荆州医院神经外科,荆州434020 [2]武汉大学人民医院神经外科

出　　处：《实用肿瘤学杂志》2024年第3期168-172,共5页Practical Oncology Journal

摘　　要：目的研究桑辛素在体外对人脑胶质母细胞瘤U87细胞迁移和侵袭能力的影响,并探讨其作用机制。方法利用细胞毒性及活性检测试剂盒(Cell counting kit-8,CCK-8)检测桑辛素对U87细胞增殖能力的影响;利用细胞划痕实验及Transwell侵袭实验检测桑辛素对U87细胞迁移和侵袭的影响;Western blot检测桑辛素对U87细胞中波形蛋白(Vimentin)、基质金属蛋白酶2(Matrix metalloproteinas,MMP-2)表达水平的影响。结果CCK-8检测结果显示,与0μg/mL的桑辛素相比,1、2、4、6μg/mL的桑辛素能够显著抑制U87细胞的增殖(P<0.001);划痕实验结果表明桑辛素浓度2、4、6μg/mL组迁移率分别为(20.597±1.225)%、(14.734±1.528)%和(7.811±1.496)%,显著低于0μg/mL组的(40.566±3.284)%(P<0.001);Transwell侵袭实验结果显示,桑辛素浓度2、4、6μg/mL组划痕愈合率分别为(85.000±6.557)、(41.000±6.245)和(13.333±3.215)个,显著低于0μg/mL组(116.667±14.572)(P<0.01);Western blot检测结果显示随着桑辛素浓度的增加,U87细胞中Vimentin的表达水平升高(P<0.001),而MMP-2的表达水平降低(P<0.001)。结论桑辛素能够有效抑制U87细胞的迁移和侵袭能力,其作用在一定的范围内与桑辛素的浓度呈正相关性。Objective The aim of this study was to investigate the effects of morusin on the migration and invasion of human glioblastoma U87 cells,and to explore its mechanism of action.Methods The Cell Counting Kit-8(CCK-8)was used to detect the effect of morusin on proliferation of U87 cells.Wound-healing and Transwell assays were used to detect the effects of morusin on migration and invasion of U87 cells.Western blot was employed to detect the effect of morusin on the expressions of MMP-2 and Vimentin in U87 cells.Results The results of CCK-8 assay showed that morusin(1,2,4,and 6μg/mL)could significantly inhibit the proliferation of U87 cells compared to the 0μg/mL morusin(P<0.001).The results of wound-healing assay showed that the migration rates of morusin-treated groups(2,4,and 6μg/mL)were(20.597±1.225)%,(14.734±1.528)%and(7.811±1.496)%,respectively,which were significant lower than that in the 0μg/mL group(40.566±3.284)%(P<0.001).The results of Transwell assay showed that the invasion number of U87 cells treated with morusin at the concentrations of 2,4,and 6μg/mL was 85.000±6.557,41.000±6.245,and 13.333±3.215,respectively,which were significant lower than that in the 0μg/mL group(116.667±14.572)(P<0.001).The results of Western blot showed that the expression of Vimentin in U87 cells increased gradually accompanying with the increase of morusin concentrations,while the expression of MMP-2 decreased gradually accompanying with the increase of morusin concentrations(P<0.001).Conclusion Morusin can effectively inhibit the migration and invasion ability of U87 cells,and its effect is positively correlated with the concentration of morusin within a certain range.

关 键 词：桑辛素 胶质母细胞瘤 迁移 侵袭 

分 类 号：R979.1[医药卫生—药品]