荧光定量PCR法与培养法检测解脲支原体结果比对分析  

Comparison of fluorescence quantitative PCR and culture detecting ureaplasma urealyticum

作　　者：刘喆[1] LIU Zhe(Medical Technical Support Department,Estern Medical Branch of PLA General Hospital,Beijing 100144,China)

机构地区：[1]解放军总医院京西医疗区医技保障科,北京100144

出　　处：《国际检验医学杂志》2024年第S01期127-129,共3页International Journal of Laboratory Medicine

摘　　要：目的通过比较荧光定量PCR法(qPCR)和培养法在解脲支原体(UU)检测中的符合率,为临床解脲支原体检测方法的选择提供参考依据。方法收集近期就诊于该院112例患者的生殖道分泌物标本,分别采用qPCR法与培养法检测UU。结果在112例标本中,UU培养法阳性53例,阳性率为47.32%,qPCR法阳性61例,阳性率54.46%(χ2=1.143,P=0.285>0.05)。61例qPCR法阳性的UU浓度范围1.60×10^(3)-1.04×10^(7)copy/mL,其中10^(3)copy/mL 12例(19.7%),10^(4)copy/mL 15例(24.6%),10^(5)copy/mL 22例(36.1%),10^(6)copy/mL 9例(14.7%),10^(7)copy/mL 3例(4.9%)。培养法阴性而qPCR法阳性病例9例,qPCR法测定浓度范围10^(3)~10^(5)copy/mL。结论qPCR法检测UU的阳性率略高于培养法,两种方法不符合结果的UU测定浓度分布在10^(3)-10^(5)copy/mL之间。Objective To compare the conformity rates of fluorescence quantitative PCR(qPCR)and culture methods in the detection of ureaplasma urealyticum(UU),a reference basis is provided for the selection of clinical detection methods for UU.Methods UU was detected from specimens 112 in Estern Medical Branch of PLA General Hospital by fluorescence quantitative and culture.Results Among 112 samples,53 samples were positive by UU culture,the positive rate was 47.32%,61 samples were positive by qPCR,and the positive rate was 54.46%(χ2=1.143,P=0.285>0.05).The concentration range of UU in 61 samples with positive qPCR was 1.60×10^(3)-1.04×10^(7)copies/ml,12 samples of 10^(3)copies/ml(19.7%),15 samples of 10^(4)copies/ml(24.6%),22 samples of 10^(5)copies/ml(36.1%),9 samples of 10^(6)copies/ml(14.7%),3 samples of 10^(7)copies/ml(4.9%).The culture method was negative and the qPCR method was positive in 9 cases.The concentration range of UU in 61 cases with positive qPCR was 1.60×10^(3)-1.04×10^(7)copies/mL.Conclusion The positive rate of UU detected by qPCR was slightly higher than that by culture.And the concentration distribution of UU in both methods was between 10^(3)and 10^(5)copies/mL.

关 键 词：荧光定量PCR法 解脲支原体 培养鉴定法 

分 类 号：R446.5[医药卫生—诊断学]