血小板抗体检测和临床应用进展综述  

Review on methods of anti-platelet antibody measurement and clinical applications

作　　者：余枝广 肖良品 吴晓丽(综述) 茅培华(审校) YU Zhiguang;XIAO Liangpin;WU Xiaoli;MAO Peihua(Medcaptain Medical Technology Co.,Ltd.,Shenzhen,Guangdong 518000,China;Shanghai Runpu Biotechnology Co.,Ltd.,Shanghai 200131,China)

机构地区：[1]深圳麦科田生物医疗技术股份有限公司,深圳518000 [2]上海润普生物技术有限公司,上海200131

出　　处：《国际检验医学杂志》2024年第S01期179-185,共7页International Journal of Laboratory Medicine

摘　　要：血小板抗体与多种临床疾病相关,具代表性的有免疫性血小板减少症如胎儿/新生儿血小板减少症,血小板输注无效,和输血后紫癜等。血小板抗体的检测方法历经变化,从传统的固相凝集反应,酶联免疫吸附试验,简易致敏红细胞血小板血清学试验,血小板抗原单抗特异性固相化法,发展至针对血小板表面抗原特异性抗体的免疫荧光和流式细胞术检测等。本文就血小板抗体分类,检测方法,临床应用及血小板抗体相关疾病的个性化治疗进行了综述。Anti-platelet antibodies are the root causes of multiple clinical illnesses,typical examples are Immune Thrombocytopenia(ITP),such as Fetal/Neonatal Alloimmune Thrombocytopenia(FNAIT),Platelet Transfusion Refractoriness(PTR),and Post-transfusion Thrombocytopenic Purpura(PTTP)et al.Measurement of anti-platelet antibodies has evolved in the past years,from traditional agglutination method,Enzyme-linked Immunosorbent Assay(ELISA),Simplified Sensitized Erythrocyte Platelet Serology Assay(SEPSA),Monoclonal Antibody Immobilization of Platelet Antigen(MAIPA),to immunofluorescence and flow cytometry detection of antibodies against specific platelet surface proteins.This article reviews the classification and detection methods of anti-platelet antibodies,the progress in clinical applications,and personalized treatment of anti-platelet antibody related diseases.

关 键 词：血小板抗体 免疫性血小板减少症 血小板输注无效 输血后紫癜 血小板抗原单抗固相化法 

分 类 号：R446.11[医药卫生—诊断学]