小鼠同种异体皮质骨锚钉植入肱骨头实验  

Allogenic cortical bone anchor implantation into humeral head in mice

作　　者：李中耀 甘露 吴毅东 于康康 袁伟 李文正 赵衷源 安明扬 李春宝 LI Zhong-yao;GAN Lu;WU Yi-dong;YU Kangkang;YUAN Wei;LI Wen-zheng;ZHAO Zhong-yuan;AN Ming-yang;LI Chun-bao(Department of Sports Medicine,The Fourth Med-ical Center,PLA General Hospital,Beijing 100048,China;Department of Orthopedics,Special Medical Center,Strategic Support Force ofPLA,Beijing 100101,China;The Fifth Clinical College,Shanxi Medical University,Taiyuan 030012,China)

机构地区：[1]中国人民解放军总医院第四医学中心运动医学科,北京100048 [2]中国人民解放军战略支援部队特色医学中心骨科,北京100101 [3]山西医科大学第五临床医学院,山西太原030012

出　　处：《中国矫形外科杂志》2024年第12期1124-1130,共7页Orthopedic Journal of China

摘　　要：[目的]将小鼠同种异体皮质骨锚钉植入肱骨头,观察检测成骨愈合情况。[方法] 54只C57BL6/J小鼠随机分为两组,27只用于制备同种异体皮质骨锚钉;另外27只为同种异体皮质骨锚钉植入受体。于植入术后1、2、4、6、8、10周各有3只动物行组织观察、免疫组化检测。1、4、10周时各有3只动物行Mcro-CT检测。[结果]组织形态方面,同种异体皮质骨锚钉植入后,同种异体皮质骨锚钉周围骨小梁逐渐增加,同种异体皮质骨锚钉逐渐被吸收,新生骨小梁长入同种异体皮质骨锚钉内部。Micro-CT检测显示,随着1周、4周和10周的时间推移,骨小梁体积显著增加[(0.20±0.37) mm^(3),(0.27±0.35) mm^(3),(0.34±0.38) mm^(3), P=0.001]。免疫组化检测显示,随植入后1、2、4、6、8和10周的时间推移,OCN [(0.48±0.05),(0.65±0.05),(0.64±0.06),(0.68±0.11),(0.73±0.03),(0.72±0.03), P=0.004]、TGF-β1 [(0.49±0.02),(0.58±0.02),(0.64±0.02),(0.67±0.01),(0.72±0.01),(1.07±0.07), P<0.001]、JNK1 [(0.51±0.02),(0.63±0.01),(0.65±0.01),(0.68±0.07),(0.71±0.10),(0.83±0.19), P=0.022]的光密度(optical density, OD)值均显著升高。[结论]小鼠同种异体皮质骨锚钉植入肱骨头可验证材料的生物学性能,证实同种异体皮质骨锚钉在宿主骨内成骨愈合能力良好。[Objective]To observe and detect the osteogenic capacity of allogenic cortical bone anchors implanted into the humeralhead in mice.[Methods]A total of 54 C57BL6/J mice were randomly divided into two groups,27 mice were used for preparing allogenic cor-tical bone anchors,while another 27 mice were used as receptors for allograft cortical bone anchors.Three animals were sacrificed for tissueobservation and immunohistochemical detection at 1 week,2 weeks,4 weeks,6 weeks,8 weeks and 10 weeks after implantation,additional-ly,for Mcro-CT at 1,4 and 10 weeks respectively.[Results]In term of histological observation,trabecular bone gradually increased aroundallogenic cortical bone anchors after implantation,while the allogenic cortical bone anchor was gradually absorbed,with new bone growinginto the interior of allogenic cortical bone anchors.Micro-CT showed that the trabecular volume increased significantly with the time of 1,4and 10 weeks[(0.20±0.37)mm^(3),(0.27±0.35)mm^(3),(0.34±0.38)mm^(3),P=0.001].Immunohistochemical assays showed that with time of 1,2,4,6,8 and 10 weeks after implantation,osteocalcin(OCN)[(0.48±0.05),(0.65±0.05),(0.64±0.06),(0.68±0.11),(0.73±.03),(0.72±0.03),P=0.004],TGF-β1[(0.49±0.02),(0.58±0.02),(0.64±0.02),(0.67±0.01),(0.72±0.01),(1.07±0.07),P<0.001],andc-Jun N-terminal kinase(1JNK1)[(0.51±0.02),(0.63±0.01),(0.65±0.01),(0.68±0.07),(0.71±0.10),(0.83±0.19),P=0.022]significantly increased in term of opticaldensity(OD)values.[Conclusion]The biological properties of mouse allogenic cortical bone anchors can be verified by implantation into hu-merus head,and the osteogenic capacity of the allogenic cortical bone anchors in host bone is proved to be good.

关 键 词：小鼠 骨锚钉 体内植入 肱骨头 成骨能力 

分 类 号：R687[医药卫生—骨科学]