基于网络药理学探讨千金藤素抗类风湿性关节炎的作用机制  

Mechanism of Cepharanthine against Rheumatoid Arthritis Based on Network Pharmacology

作　　者：王晨屹 孙广臣[1] Wang Chenyi;Sun Guangchen(Guilin Medical University,Guilin 541199,China)

机构地区：[1]桂林医学院,广西桂林541199

出　　处：《广东化工》2024年第11期53-55,46,共4页Guangdong Chemical Industry

基　　金：广西自然科学基金项目(2018GXNSFAA138072)。

摘　　要：目的:本研究通过网络药理学筛选千金藤素(CEP)治疗类风湿性关节炎(RA)的相关靶点并探索其潜在作用机制。方法:首先,使用Swiss Target Prediction和DrugBank数据库预测CEP潜在的作用靶点,使用Disgenet数据库、Gene Cards数据库和NCBI数据库获取RA相关靶点。然后使用Venny2.1筛选CEP潜在的作用靶点和RA相关靶点交集。将共有靶点导入STRING数据库获得PPI网络。使用Cytoscape对PPI网络进行可视化并筛选关键基因。使用DAVID数据库对关键基因进行GO和KEGG富集分析。结果:筛选出CEP潜在的作用靶点101个,RA相关靶点共2196个。筛选出56个共有基因和7个关键基因:JAK2、PRKCB、SRC、MTOR、AKT1、PI3K、EGFR。对关键基因进行GO和KEGG富集分析,GO分析的BP富集于表皮生长因子受体信号通路、蛋白激酶B信号的正调节,巨自噬的负调节、一氧化氮生物合成过程的正调节和细胞周期蛋白依赖性蛋白质丝氨酸/苏氨酸激酶活性的正调节等;CC富集于细胞质中;MF富集于蛋白激酶活性、激酶活性、ATP结合、蛋白激酶C结合、蛋白质丝氨酸/苏氨酸激酶活性以及蛋白激酶结合。KEGG主要富集于抗癌通路、抗炎通路和抗病毒途径。结论:本研究简要分析了千金藤素抗RA的潜在靶点和潜在作用机制,为进一步探索千金藤素抗RA的潜在保护机制提供了理论依据。Objective:The aim of this study was to screen relevant targets and explore the potential mechanism of action of cepharanthine(CEP)for treating rheumatoid arthritis(RA)using network pharmacology.Methods:The study utilised the SwissTargetPrediction and DrugBank to predict potential targets for CEP.Additionally,the Disgenet,GeneCards,and NCBI were consulted to obtain RA-related targets.Potential targets for CEP and RA-related target intersections were then screened using Venny 2.1.The shared targets were imported into the STRING database to obtain the PPI network.The PPI network was visualised and screened for key genes using Cytoscape.Key genes were analysed for GO and KEGG enrichment using the DAVID database.Results:A total of 2196 targets related to RA were screened,along with 101 potential targets of action for CEP.56 common genes and 7 key genes were identified:JAK2,PRKCB,SRC,MTOR,AKT1,PI3K,and EGFR.The study conducted GO and KEGG enrichment analyses on key genes.The GO analysis revealed enrichment in the epidermal growth factor receptor signalling pathway,positive regulation of protein kinase B signalling,negative regulation of macroautophagy,positive regulation of nitric oxide biosynthesis process,and cell cycle protein-dependent protein serine/threonine kinase activity.The cytoplasm showed an enrichment of CC.Protein kinase activity,kinase activity,ATP binding,protein kinase C binding,protein serine/threonine kinase activity,and protein kinase binding were enriched in MF.KEGG was mainly enriched in the anticancer pathway,anti-inflammatory pathway,and antiviral pathway.Conclusion:this study provides a brief analysis of the potential targets and mechanisms of action of cepharanthine anti-RA.It offers a theoretical basis for further exploration of the potential protective mechanisms of cepharanthine anti-RA.

关 键 词：千金藤素 类风湿关节炎 网络药理学 作用机制 抗炎 

分 类 号：TQ[化学工程]