两株头孢菌素高效降解菌的筛选及其在污水处理中的应用  

Selection of Two Degrading Bacteria Associated with Cephalosporin and Its Application in Sewage Treatment

作　　者：王华光 郭旭 王婷 Wang Huaguang;Guo Xu;Wang Ting(Shandong Kaifengyuan Environmental Technology Company,Yantai 264000,China)

机构地区：[1]山东凯丰源环境科技有限公司,山东烟台264000

出　　处：《广东化工》2024年第12期123-126,共4页Guangdong Chemical Industry

摘　　要：近年来,由于头孢菌素类药物的广泛使用,头孢菌素类药物的残留问题日益严重,获得头孢菌素高效降解菌将有助于解决社会生产生活过程中产生的头孢菌素及其残留物造成的污染问题。为获得高效降解头孢菌素菌的降解菌,采取烟台市污水处理厂的活性污泥,将活性污泥放置于单室微生物燃料电池,在含有活性污泥的阳极室中添加头孢氨苄抗生素,驯化活性污泥中的微生物。添加头孢氨苄后,活性污泥细菌群落结构发生了变化,Mariniradius属和水单胞菌属的细菌相对丰度增加。利用添加头孢氨苄抗生素的选择性培养基,筛选到两株可降解头孢氨苄抗生素的菌株:隶属于的色杆菌属OT1菌(Achromobactersp.)和隶属于的苍白杆菌属OT2菌(Ochrobactrumsp.),降解菌对头孢氨苄的降解效率受到温度的影响,两株降解菌株在高盐度环境下提高对头孢氨苄的降解效率;另外Achromobacter sp.和Ochrobactrum sp.增强了活性污泥降解头孢氨苄和化学需氧量(CODCr)的去除率。筛选头孢氨苄的高效降解菌株,分析环境因子对降解菌降解效率的影响,将为降解菌在抗生素去除过程中的实际应用提供数据参考。With the extensive use of cephalosporins in recent years,cephalosporins and their residues in the environment caused many environmental problems.The degrading bacteria would solve cephalexin pollutions caused by the social production.To obtain the degrading bacteria,cephalexin was added into in the anode of microbial fuel cells including the activated sludge from a sewage-treatment plant of Yantai to domesticate bacterial community.High-throughput sequencing technology determined the changes of bacterial community composition before and after adding the cephalexin into activated sludge using 16S rDNA sequence.Mariniradius and Aquimonas increased their relative abundances.Two degrading strains Achromobacter sp.and Ochrobactrum sp.were isolated from carbon cloth electrode by the selective medium including cephalexin.Temperature affected the removal efficiency of cephalexin,and removal efficiencies on the cephalexin by two strains were enhanced by salinity.Two strains degrading bacteria enhanced the removal efficiencies of activated sludge on the cephalexin and chemical oxygen demand.The study of degradation will provide data for the application of microbial degradation in the removal of cephalexin.

关 键 词：头孢菌素 降解菌 盐度 温度 活性污泥 

分 类 号：TQ[化学工程]