莲子心中具有心肌细胞保护活性的化学成分研究  

Chemical constituents from Nelumbinis Plumula and their protective effects on cardiac muscle cell

作　　者：李佳东 高晗 李英哲 张贵兰 吴弢[1] LI Jia-dong;GAO Han;LI Ying-zhe;ZHANG Gui-lan;WU Tao(Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学中药研究所,上海201203

出　　处：《天然产物研究与开发》2024年第7期1158-1165,1181,共9页Natural Product Research and Development

基　　金：国家自然科学基金(81973475)。

摘　　要：为了寻找莲子心(Nelumbinis Plumula)具有心肌细胞保护活性的化学成分,本研究采用多种柱色谱技术分离和纯化莲子心的80%乙醇提取物,运用^(1)H NMR、^(13)C NMR等波谱技术和文献数据比对鉴定化合物结构,并对化合物进行心肌细胞保护活性筛选。从莲子心80%乙醇提取物分离纯化得到14个化合物,分别鉴定为柚皮苷(1)、山柰酚-3-O-β-D-芸香糖苷(2)、芹菜素-6-C-β-L-阿拉伯糖-8-C-β-D-葡萄糖苷(3)、木犀草素-7-O-β-D-新橙皮苷(4)、木犀草素-3′-O-β-D-葡萄糖苷(5)、芹菜素-7-O-β-D-葡萄糖苷(6)、橙皮苷(7)、异夏佛塔苷(8)、松脂素-4-O-β-D-葡萄糖苷(9)、对香豆酸-4-O-β-D-葡萄糖苷(10)、对香豆酸(11)、苯乙基6-O-α-L-呋喃阿拉伯糖基-β-D-葡萄糖苷(12)、淫羊藿次苷D1(13)、淫羊藿次苷F2(14)。化合物1~6、9、11~14均为首次从莲子心中分离得到。黄酮类化合物1~8对缺氧/复氧损伤下的小鼠HL-1心肌细胞具有明显的保护作用,对小鼠HL-1-STAT3-Luc细胞中STAT3的表达也具有促进作用,且均具有剂量依赖性。This study aims to investigate the chemical constituents from Nelumbinis Plumula and their protective effects on cardiac muscle cell.Fourteen compounds were isolated and purified from 80% ethanol extract by various column chromatography techniques,and identified by ^(1)H NMR,^(13)C NMR and other spectroscopic techniques.The compounds were screened for cardiomyocyte protective activity.Fourteen compounds were identified as naringin(1),kaempferol-3-O-β-D-rutinoside(2),apigenin-6-C-β-L-arabinopyranosyl-8-C-β-D-glucopyranoside(3),luteolin-7-O-β-D-neohesperidoside(4),luteolin-3′-O-β-D-glucopyranoside(5),apigenin-7-O-β-D-glucopyranoside(6),hesperidin(7),isoschaftoside(8),pinoresinol-4-O-β-D-glucopyranoside(9),p-coumaric acid-4-O-β-D-glucopyranoside(10),p-coumaric acid(11),phenethyl-6-O-α-L-arabinfuranosyl-β-D-glucopyranoside(12),icariside D1(13),icariside F2(14).Compounds 1-6,9,and 11-14 were firstly isolated from Nelumbinis Plumula.Flavonoids compounds 1-8 showed significant protective effects against hypoxia/reoxygenation injury in HL-1 cardiomyocytes,as well as promoting effects on STAT3 expression in HL-1-STAT3-Luc cells in dose-dependent manner.

关 键 词：莲子心 黄酮苷 心肌细胞保护作用 缺氧/复氧损伤 

分 类 号：R284.2[医药卫生—中药学]