桂林会仙湿地真菌Talaromyces sp.W21的化学成分及其抗炎活性研究  

Chemical constituents and anti-inflammatory activity of Talaromyces sp.W21 from Huixian Wetland in Guilin

作　　者：梁琳 袁玺 冼小雅 周先丽[2] 王力生 邝彤东 梁成钦[1] LIANG Lin;YUAN Xi;XIAN Xiao-ya;ZHOU Xian-li;WANG Li-sheng;KUANG Tong-dong;LIANG Cheng-qin(School of Pharmacy,Guilin Medical University;School of Intelligent Medicine&Biotechnology,Guilin Medical University;Guangxi Key Laboratory of Diabetic Systems Medicine,Guilin Medical University,Guilin 541199,China)

机构地区：[1]桂林医学院药学院 [2]桂林医学院智能医学与生物技术学院 [3]桂林医学院广西糖尿病系统医学重点实验室,桂林541199

出　　处：《天然产物研究与开发》2024年第7期1175-1181,共7页Natural Product Research and Development

基　　金：国家自然科学基金(81860621)。

摘　　要：研究桂林会仙湿地来源真菌Talaromyces sp.W21的化学成分及其抗炎活性。综合运用多种柱色谱、现代波谱技术和理化性质对Talaromyces sp.W21发酵提取物中的化学成分进行分离、纯化和结构鉴定,并从中分离得到10个化合物,分别鉴定为cytospone K(1)、(3 S,4 R,5 R)-4-hydroxymethyl-3,5-dimethyldihydro-2-furanone(2)、lasdiplactone(3)、leptosphaerone C(4)、annularin A(5)、annularin K(6)、cladobotrin V(7)、2-(2′S-hydroxypropyl)-5-methyl-7-hydroxychromone(8)、bacillisporin A(9)、bacillisporin B(10)。其中,化合物1为新的α-吡喃酮类化合物,化合物2~8为首次从Talaromyces sp.中分离获得。抗炎活性实验结果表明化合物9和10对LPS诱导的RAW 264.7释放NO具有一定的抑制作用,其IC 50分别为38.90±0.79、35.02±1.03μmol/L。This study aims to investigate the chemical constituents and anti-inflammatory activity of Talaromyces sp.W21 from the Huixian wetland in Guilin.A new α-pyrone derivative,namely cytospone K(1),together with nine known compounds,namely(3 S,4 R,5 R)-4-hydroxymethyl-3,5-dimethyldihydro-2-furanone(2),lasdiplactone(3),leptosphaerone C(4),annularin A(5),annularin K(6),cladobotrin V(7),2-(2′S-hydroxypropyl)-5-methyl-7-hydroxychromone(8),bacillisporin A(9)and bacillisporin B(10)were isolated and purified from cultures of Talaromyces sp.W21 by using various column chromatography.Their structures were identified by modern spectroscopic techniques and physicochemical properties.Compound 1 was a new α-pyranone derivative,and compounds 2-8 were isolated from the Talaromyces sp.for the first time.The anti-inflammatory activity assay showed that compounds 9 and 10 have a moderate inhibition of NO production induced by LPS in RAW 264.7 cells,with IC 50 values of 38.90±0.79 and 35.02±1.03μmol/L respectively.

关 键 词：湿地真菌 化学成分 α-吡喃酮 抗炎活性 

分 类 号：R284.1[医药卫生—中药学]