柴胡-白芍单味、药对配方颗粒与传统汤剂化学成分及抗抑郁对比研究  

Comparative study on the chemical constituents and anti-depression of Bupleurum Radix-Paeoniae Radix Alba single flavor,drug-pair formula granules and traditional decoction

作　　者：杨新雪 梁进京 董蕊[1] YANG Xin-xue;LIANG Jin-jing;DONG Rui(College of Chinese Materia Medica,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学中药材学院,长春130118

出　　处：《天然产物研究与开发》2024年第7期1182-1190,共9页Natural Product Research and Development

基　　金：国家中药标准化项目(ZYBZH-Y-ZL-25)。

摘　　要：运用高效液相色谱(HPLC)法比较柴胡-白芍单味、药对配方颗粒与传统汤剂的主要有效成分变化,建立利血平拮抗急性抑郁模型探讨药对合煎与单煎合并的抗抑郁作用差异。采用COSMOSIL 5C 18-MS-Ⅱ柱(250 mm×4.6 mm,5μm);以乙腈-0.1%磷酸溶液为流动相;体积流量1.0 mL/min;柱温30℃;进样量10μL;采用双波长(210、230 nm)检测。酶联免疫吸附剂实验及对相关组织进行HE、Nissl染色分析不同剂型对小鼠抑郁效果的影响。结果表明,柴胡皂苷a、柴胡皂苷d、芍药苷和氧化芍药苷的含量在柴胡-白芍单味配方颗粒混溶中低于柴胡-白芍药对配方颗粒与传统汤剂;柴胡-白芍单味配方颗粒混溶组、柴胡-白芍药对配方颗粒组和传统汤剂组均能使小鼠脑内5-羟色胺、多巴胺水平明显提高(P<0.05,P<0.01);血清中白细胞介素-6、白细胞介素-1β及肿瘤坏死因子含量显著降低(P<0.05,P<0.01),抗炎因子白细胞介素-10显著升高(P<0.05,P<0.01);肝组织中超氧化物歧化酶不同程度地升高,丙二醛显著降低(P<0.05,P<0.01)。HPLC检测方法重复性好,稳定可靠。通过抑制炎症和缓解氧化应激,柴胡-白芍药对配方颗粒与传统汤剂较柴胡-白芍单味配方颗粒混溶改善抑郁症状显著。This study amis to compare the changes in the major active constituents of Bupleurum Radix-Paeoniae Radix Alba(BR-PR)single flavor,drug-pair formula granules and traditional decoction using high performance liquid chromatography(HPLC).The difference in antidepressant effects between the combined decoction and single decoction of drug pairs was investigated using a model of acute depression antagonised by reserpine.A COSMOSIL 5C 18-MS-II column(250 mm×4.6 mm,5μm)was used;acetonitrile(A)-0.1% phosphoric acid solution(B)was used as the mobile phase;the volume flow rate was 1.0 mL/min;The column temperature was 30℃;the injection volume was 10μL;and the detection was carried out at dual wavelengths(210 and 230 nm).The effects of different dosage forms on depression in mice were analyzed by enzyme linked immunosorbent assay kit and HE and Nissl staining.The contents of saikosaponin a,saikosaponin d,paeoniflorin,paeoniflorin oxide in BR-PR single flavor formula granules mixed solution were lower than BR-PR drug-pair formula granules and traditional decoction.The levels of 5-hydroxytryptamine and dopamine(P<0.05,P<0.01)were significantly increased in the BR-PR single flavor formula granules mixed solution group,BR-PR drug-pair formula granules group,and traditional decoction group;The contents of interleukin-6,interleukin-1βand tumor necrosis factor-αin serum significantly decreased(P<0.05,P<0.01),the anti-inflammatory factor interleukin-10 was significantly increased(P<0.05,P<0.01);The superoxide dismutase was increased,while malondialdehyde significantly decreased in liver tissues(P<0.05,P<0.01).HPLC method has good repeatability,stability and reliability.By inhibiting inflammation and relieving oxidative stress,BR-PR drug-pair formula granules and traditional decoction improved depressive symptoms significantly than BR-PR single flavor formula granules mixed solution.

关 键 词：柴胡-白芍 配方颗粒 传统汤剂 主要成分 抗抑郁 

分 类 号：R283.6[医药卫生—中药学]