亚慢性PM_(2.5)和O_(3)共同暴露对大鼠鼻黏膜ATP总量及ATP酶活性的影响  

Effects of PM_(2.5) and O_(3) sub-chronic combined exposure on ATP amount and ATPase activities in rat nasal mucosa

作　　者：阎腾龙 胥嘉钰 陈田 杨鑫 王伟伟[4] 周淑佩 牛丕业 贾光[1] 夏交[4] YAN Tenglong;XU Jiayu;CHEN Tian;YANG Xin;WANG Weiwei;ZHOU Shupei;NIU Piye;JIA Guang;XIA Jiao(Department of Occupational and Environmental Health Sciences,Peking University School of Public Health,Beijing 100191,China;Beijing Institute of Occupational Disease Prevention and Treatment,Beijing 100093,China;School of Public Health and the Beijing Key Laboratory of Environmental Toxicology,Capital Medical University,Beijing 100069,China;Department of Otorhinolaryngology,Head and Neck Surgery,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;Department of Laboratory Animal Science,Peking University Health Science Center,Beijing 100191,China)

机构地区：[1]北京大学公共卫生学院劳动卫生与环境卫生学系,北京100191 [2]北京市职业病防治研究院,北京100093 [3]首都医科大学公共卫生学院,首都医科大学环境毒理学北京市重点实验室,北京100069 [4]首都医科大学附属北京友谊医院耳鼻咽喉头颈外科,北京100050 [5]北京大学医学部实验动物科学部,北京100191

出　　处：《北京大学学报（医学版）》2024年第4期687-692,共6页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金(91643111、91743114)。

摘　　要：目的:探讨细颗粒物(fine particle matter,PM_(2.5))和臭氧(ozone,O_(3))亚慢性共同暴露对大鼠鼻黏膜组织腺嘌呤核苷三磷酸(adenosine triphosphate,ATP)总量及ATP酶活性的影响。方法:采用随机数字表法将20只雄性Sprague Dawley(SD)大鼠均分为对照组和暴露组,每组各10只,分别饲养于常规清洁级环境和本团队既往所搭建的大气污染物暴露系统中,连续暴露208 d。暴露期间,监测暴露系统内PM_(2.5)和O_(3)浓度,采用自测和站点数据相结合的方法对暴露系统内PM_(2.5)和O_(3)进行综合评估。在暴露第208天处死大鼠取心、肝、脾、肾、睾丸等主要器官和鼻黏膜组织,称量各脏器重量并计算脏器系数。利用所收集鼻黏膜组织,使用生物发光法测定ATP总量,使用分光光度法检测Na^(+)-K^(+)-ATP酶和Ca^(2+)-ATP酶活性。使用两独立样本t检验比较各指标组间差异。结果:自第3周开始至暴露结束,暴露组大鼠体质量高于对照组(P<0.05),两组间脏器系数差异无统计学意义。暴露组日均PM_(2.5)浓度为(30.68±19.23)μg/m3,O_(3)最大8 h浓度(O_(3)-8 h)为(82.45±35.81)μg/m3。暴露组大鼠鼻黏膜组织ATP化学发光值(792.4±274.1)IU/L低于对照组(1126.8±218.1)IU/L,鼻黏膜组织Na^(+)-K^(+)-ATP酶活性(1.53±0.85)U/mg低于对照组(4.31±1.60)U/mg(P<0.05)。对照组和暴露组鼻黏膜组织的蛋白含量分别为(302.14±52.51)mg/L和(234.58±53.49)mg/L,Ca^(2+)-ATP酶活性分别为(0.81±0.27)U/mg和(0.99±0.73)U/mg,组间差异均无统计学意义。结论:亚慢性PM_(2.5)和O_(3)共同暴露可能影响大鼠鼻黏膜组织供氧能力。Objective:To evaluate the effects of fine particle matter(PM_(2.5))and ozone(O_(3))combined exposure on adenosine triphosphate(ATP)amount and ATPase activities in nasal mucosa of Sprague Dawley(SD)rats.Methods:Twenty male SD rats were divided into control group(n=10)and exposure group(n=10)by random number table method.The rats were fed in the conventional clean environment and the air pollutant exposure system established by our team,respectively,and exposed for 208 d.During the exposure period,the concentrations of PM_(2.5)and O_(3)in the exposure system were monitored,and a comprehensive assessment of PM_(2.5)and O_(3)in the exposure system was conducted by combining self-measurement and site data.On the 208 d of exposure,the core,liver,spleen,kidney,testis and other major organs and nasal mucosal tissues of the rats were harvested.Each organ was weighed and the organ coefficient calculated.The total amount of ATP was measured by bioluminescence,and the activities of Na^(+)-K^(+)-ATPase and Ca^(2+)-ATPase were detected by spectrophotometry.The t test of two independent samples was used to compare the differences among the indicator groups.Results:From the 3rd week to the end of exposure duration,the body weight of the rats in the exposure group was higher than that in the control group(P<0.05),and there was no significant difference in organ coefficients between the two groups.The average daily PM_(2.5)concentration in the exposure group was(30.68±19.23)μg/m^(3),and the maximum 8 h ozone concentration(O_(3)-8 h)was(82.45±35.81)μg/m^(3).The chemiluminescence value(792.4±274.1)IU/L of ATP in nasal mucosa of the rats in the exposure group was lower than that in the control group(1126.8±218.1)IU/L.The Na^(+)-K^(+)-ATPase activity(1.53±0.85)U/mg in nasal mucosa of the rats in the exposure group was lower than that in the control group(4.31±1.60)U/mg(P<0.05).The protein content of nasal mucosa in the control group and the exposure group were(302.14±52.51)mg/L and(234.58±53.49)mg/L,respectively,and the acti

关 键 词：细颗粒物 臭氧 腺嘌呤核苷三磷酸 

分 类 号：R122.7[医药卫生—环境卫生学]