叶尔羌河土著鱼类寄生对盲囊线虫的分子鉴定和群体遗传结构分析  

Molecular identification and population genetic structure analysis of the parasitic Contracaecum sp.in indigenous fishes of the Yarkant River

作　　者：王瑾璞 石彩霞 刘彦君 张丽 魏念文 岳城[1] 郝翠兰[1] WANG Jinpu;SHI Caixia;LIU Yanjun;ZHANG Li;WEI Nianwen;YUE Cheng;HAO Cuilan(College of Veterinary Medicine,Xinjiang Agricultural University/Xinjiang Key Laboratory of New Drug Study and Creation for Herbivorous Animals,Urumqi 830052,China)

机构地区：[1]新疆农业大学动物医学学院/新疆草食动物新药研究与创制重点实验室,乌鲁木齐830052

出　　处：《淡水渔业》2024年第4期49-56,共8页Freshwater Fisheries

基　　金：新疆维吾尔自治区重点研发任务专项(2023B02012);国家自然科学基金项目(31960737);新疆维吾尔自治区自然科学基金项目(2021D01B56)。

摘　　要：为探明叶尔羌河5种土著鱼寄生对盲囊线虫(Contracaecum sp.)的群体遗传结构,基于其核糖体ITS-1基因进行分子鉴定,并利用线粒体COⅡ基因进行遗传结构分析。结果显示,5个对盲囊线虫群体均为鲁道夫对盲囊线虫B种(Contracaecum rudolphii B),共检测出71个单倍型,5个群体的单倍型多样性为0.714~0.978,核苷酸多样性为0.00540~0.01091。系统发育树和网络图显示,5个群体间未形成与宿主相对应谱系。遗传分化指数为-0.0013~0.1086,AMOVA分析显示,群体间变异和群体内变异分别占4.33%和95.67%,5个群体遗传变异主要来自群体内部。综上,叶尔羌河5种土著鱼体内寄生鲁道夫对盲囊线虫B种群体均属高遗传多样性群体,各群体间分化程度较低,群体间基因交流频繁。The present study was carried out by a previous In this study,species identification,inter-population genetic distance and variation to investigate the population genetic structure of five indigenous fish species of Schizothorax biddulphi,S.labrosus,Diptychus maculatus,Triplophysa tenuis and T.stoliczkae parasitizing Contracaecum sp.within the Yarkand River Basin.Meanwhile,population structure were analyzed by the ribosomal ITS-1 gene and mitochondrial COⅡgene of a total of 112 Contracaecum sp.hosts.The comparison results of the evolutionary tree constructed by the neighbour-joining method showed thatall the five host fish species of Contracaecum sp.were Contracaecum rudolphii B based on the homology analysis of the ribosomal ITS-1 gene in NCBI website.A total of 71 haplotypes were detected based on the COⅡ gene;one shared haplotype was Hap6,and the other was Hap30,which was only Schizothoracine.Hap30 occurred only in the Schizothorax population.The diversity analysis showed that the haplotype diversity(hd)of the five populations ranged from 0.714 to 0.978.The nucleotide diversity(Pi)ranged from 0.00540 to 0.01091.Among them,the Hd and Pi of the T.stoliczkae population were the lowest,with the values of 0.714 and 0.00540;and those of the D.maculatus population were the highest,with the values of 0.978 and 0.01091.The neighboring population had the highest values,with values of 0.978 and 0.978,while the neighboring population had the highest values.Neighbor-joining methods of constructing evolutionary trees and haplotype network diagrams showed no lineage corresponding to the host was formed among the five populations.The genetic differentiation indices ranged from-0.0013 to 0.1086.Population genetic structure and AMOVA analyses showed that the five populations were weakly differentiated and had small genetic distances,with inter-population and intra-population variation accounting for 4.33% and 95.67%,respectively.The five populations mainly derived their genetic variation within the populations.It was conclu

关 键 词：鲁道夫对盲囊线虫(Contracaecum rudolphii) ITS-1序列 COⅡ基因 遗传结构 

分 类 号：S941.5[农业科学—水产养殖]