陆地棉自然早落叶突变系的激素含量分析  

Analysis of Hormone Content in Naturally Early Deciduous Mutant Line of Upland Cotton

作　　者：陈旭升[1] 赵亮[1] 狄佳春[1] CHEN Xusheng;ZHAO Liang;DI Jiachun(Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Nanjing,Jiangsu 210014,China)

机构地区：[1]江苏省农业科学院经济作物研究所,江苏南京210014

出　　处：《作物研究》2024年第3期202-204,217,共4页Crop Research

基　　金：国家转基因生物新品种培育科技重大专项子课题(2016ZX08005001-008)。

摘　　要：为研究陆地棉自然早落叶性状的激素表达特征,本研究采用自然早落叶突变系落叶棉501为试验材料,以叶片功能期长的近等基因系N065、陆地棉品系N002作为对照1与对照2,采用酶联免疫法(ELISA)定量测定不同组织的脱落酸(ABA)、赤霉素(GA3)、玉米素(ZR)和生长素(IAA)含量。从种胚激素含量看,落叶棉501的ABA含量极显著高于对照,分别是对照1与对照2的2.86倍和2.39倍;其GA3含量极显著低于对照,仅为对照1、对照2的21.4%、23.6%;其ZR含量也显著低于对照,分别为对照1、对照2的70.7%、60.4%;但其IAA含量与对照无显著差异。从叶片激素含量看,落叶棉501的ABA含量极显著高于对照,分别是对照1与对照2的2.65倍和5.63倍;其GA3含量则极显著低于对照,分别为对照1与对照2的21.8%、31.8%;其ZR含量与对照无显著差异;IAA的含量则显著低于对照,分别为对照1、对照2的21.7%和31.6%。综合分析,陆地棉早落叶性状与种胚、叶片中的ABA及GA3含量存在明显关系,可根据陆地棉顶端小真叶或种胚中的ABA、GA3含量,初步预判其是否具有早落叶特性。In order to study the characteristics of hormone content expression of naturally early defoliation traits in upland cotton,a naturally early deciduous mutant line Defoliation cotton 501 was used as experimental material,and the near-isogenic line N065 and upland cotton line N002 with long leaf function stage were used as control 1 and control 2,respectively.The contents of ABA,GA3,ZR and IAA in different tissues were determined by Enzyme-Linked Immunosorbent Assay.In terms of seed embryo hormone content,the ABA content of deciduous cotton 501 was significantly higher than that of controls,only 70.7% and 60.4% of control 1 and control 2,respectively,and its GA3 content was significantly lower than that of control 1 and control 2,which was only 21.4% and 23.6% of that of control 1 and control 2,respectively.Its ZR content was also significantly lower than that of control 1 and control 2,respectively,but there was no significant difference in IAA content between control 1 and control 2.In terms of leaf hormone content,the ABA content of deciduous cotton 501 was significantly higher than controls,that was 2.65 and 5.63 times of control 1 and control 2,respectively,while its GA3 content was significantly lower than that of control 1 and control 2,21.8% and 31.8% of that of control 1 and control 2,respectively.There was no significant difference in ZR content between the control and the control,while the IAA content was significantly lower than that of controls only 21.7% and 31.6% control 1 and control 2,respectively.Comprehensive analysis showed that the early defoliation character of upland cotton was significantly related to the content of ABA and GA3 in seed embryo and leaf.According to the content of ABA and GA3 in apical true leaf or seed embryo of upland cotton,the early defoliation characteristic of upland cotton could be predicted.

关 键 词：陆地棉 自然早落叶 突变系 激素 ABA GA 

分 类 号：S562.01[农业科学—作物学]