月季黑斑病的病原特征及其对杀菌剂的敏感性分析  

Pathogenic Characteristics of Rose Black Spot and Its Sensitivity to Fungicides

作　　者：周锋 安家琳 苏海川 张胜 王国权 逯盼盼 刘润强[1,2] ZHOU Feng;An Jia-lin;SU Hai-chuan;ZHANG Sheng;WANG Guo-quan;LU Pan-pan;LIU Run-qiang(School of Resources and Environment,Henan Institute of Science and Technology/Henan Engineering Technology Research Center of Green Pesticide Creation and Intelligent Sensor Monitoring,Xinxiang 453003,China;Baiquan Institute of Moderm Agricultural Technology,Xinxiang 453003,China)

机构地区：[1]河南科技学院资源与环境学院/河南省绿色农药创制与智能传感监测工程技术研究中心,河南新乡453003 [2]河南科技学院百泉现代农业研究院,河南新乡453003

出　　处：《河北农业科学》2024年第3期57-61,共5页Journal of Hebei Agricultural Sciences

基　　金：河南省中央引导地方科技发展项目(Z20221343034);河南省重大科技专项(221100110100);河南科技学院大学生创新创业训练计划项目(2023CY039)。

摘　　要：月季是重要的观赏性植物,病害发生后会严重影响月季的外观品质。利用组织分离法,从月季病叶样品中分离和纯化了候选菌株;对供试菌株进行形态学鉴定、rDNA-ITS分子学鉴定以及系统进化关系分析,此外,还对供试菌株进行了柯赫氏法则验证及其对常见杀菌剂的敏感性分析试验。结果表明:供试菌株的菌落及分生孢子形态与链格孢菌(Alternaria spp.)一致,rDNA-ITS序列及系统发育树分析表明供试菌株为狭卵链格孢菌(A.angustiovoidea)。同时,柯赫氏法则试验表明,供试菌株的形态特征及其对寄主月季的致病性与狭卵链格孢菌(A.angustiovoidea)完全一致。此外,供试菌株对10种常用杀菌剂敏感性的分析试验进一步表明,供试菌株对丙硫菌唑不敏感;氟啶胺、咯菌腈、咪鲜胺、氟环唑、己唑醇、苯醚甲环唑、戊唑醇、粉唑醇、丙环唑对供试菌株具有较高的抑菌活性,EC50为0.059~0.962μg/mL。研究结果为月季黑斑病的识别及其化学防控研究提供了数据支撑。Rose is an important ornamental plant,and the occurrence of diseases can seriously affect the appearance quality of rose.Candidate strains were isolated and purified from rose diseased leaf samples using tissue separation method.Morphological identification,rDNA-ITS molecular identification,and phylogenetic analysis were performed on the strains.In addition,Koch’s rule validation and sensitivity analysis of the strains to common fungicides were also conducted.The results showed that the colony and spore morphology of the tested strains were consistent with Alternaria spp.,and rDNA ITS sequence and phylogenetic tree analysis indicated that the tested strains were A.angustifolia.Meanwhile,the Koch’s rule experiment showed that the morphological characteristics of the tested strains and their pathogenicity to rose were completely consistent with those of A.angustifolia.In addition,the analysis of the sensitivity of the tested strains to 10 commonly used fungicides further showed that the tested strains were not sensitive to propiconazole.Fluaziridine,chloramphenicol,prochloraz,fluconazole,hexazole,difenoconazole,tebuconazole,fluconazole,and propiconazole had high anti-fungus activity against the tested strains,with an EC50 of 0.059-0.962μg/mL.The research results provide data support for the identification and chemical prevention and control of rose black spot.

关 键 词：黑斑病 月季 形态学特征 rDNA-ITS序列 杀菌剂敏感性 室内毒力 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]