阴囊热应激对山羊血睾屏障结构和功能的影响  

Effect of Scrotal Hyperthermia on Structure and Function of Blood-testis Barrier in Goats

作　　者：武永杰[1] 徐英环 刘腾飞 马琳[1] 陈鸿 徐永平[1] WU Yongjie;XU Yinghuan;LIU Tengfei;MA Lin;CHEN Hong;XU Yongping(College of Veterinary Medicine,Northwest A&F University,Yangling 712100,China)

机构地区：[1]西北农林科技大学动物医学院,杨凌712100

出　　处：《畜牧兽医学报》2024年第7期2973-2982,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金青年项目(31902223);陕西省自然科学基金青年项目(2021JQ-159)。

摘　　要：旨在探究热应激对山羊血睾屏障(BTB)结构和功能的影响,为山羊热应激防治提供理论依据。本研究选择6只1~1.5岁的健康雄性关中山羊,随机分为2组,每组3只。对照组正常饲喂,试验组采用改良后的阴囊热刺激法建立山羊睾丸热应激模型。利用CASA法检测精液质量变化,光镜与透射电镜观测睾丸结构,生物素示踪法显示BTB通透性改变,蛋白质印记法与免疫组织化学法检测BTB关键蛋白表达定位。热应激组中山羊睾丸指数、精子数量、精子活率、精子总运动性和精子前进运动率均显著降低(P<0.05)。热应激导致睾丸组织形态受损明显,生精细胞数量减少且排列紊乱;支持细胞内质网扩张,并出现大量空泡;支持细胞间紧密连接结构松散且不连续。热应激引起BTB通透性升高,关键连接蛋白表达水平显著降低(P<0.05),但分布定位未受影响。热应激损伤山羊睾丸组织形态,破坏支持细胞结构并降低BTB关键蛋白表达,从而导致BTB结构和功能完整性受损,造成精子质量下降,影响雄性山羊繁殖性能。The aim of this study was to investigate the effects of heat stress on the structure and function of blood-testis barrier(BTB)in goats,and to provide a theoretical basis for the prevention and treatment of heat stress in goats.Six healthy male Guanzhong goats aged 1 to 1.5 years old were selected and randomly divided into two groups with three goats in each group.Goats in the control group were fed normally,and the heat stress model was established in the experimental group by the modified scrotal heat stimulation method.The semen quality was detected by CASA,the testicular structure was observed by light microscopy and transmission electron microscopy,the permeability of BTB was detected by biotin tracer,and the expression and localization of key proteins of BTB were detected by Western blot and immunohistochemistry.In heat stress group,the testis index,spermatozoa count,spermatozoa viability,spermatozoa total motility and progressive motility were significantly decreased(P<0.05).Heat stress caused obvious morphological damage of the testis,and the number of spermatogenic cells decreased and the arrangement was disordered.Endoplasmic reticulum of sertoli cells was expanded and a large number of vacuoles appeared.The tight junction between sertoli cells was loose and discontinuous.The permeability of BTB increased,and the expression level of key junction proteins decreased significantly(P<0.05),but the distribution and location of BTB were not affected in heat stress group.Heat stress damages the morphology of goat testis,destroys the structure of sertoli cells and reduces the expression of key BTB proteins,thereby impairing the structural and functional integrity of BTB,resulting in the decline of spermatozoa quality and affect the reproductive capacity of male goats.

关 键 词：山羊 热应激法 血睾屏障 超微结构 

分 类 号：S827.3[农业科学—畜牧学]