宁夏部分地区犊牛腹泻样本大肠杆菌分离菌株的毒力因子检测与分析  

Toxicity Factor Detection and Analysis of Escherichia coli Isolated Strains from Calf Diarrhea Samples in Some Areas of Ningxia

作　　者：王玮 王景松[1,2] 郭亚男 高乐 王玲玲 陈灿[2] 王健霖 王建东 马科 李继东 WANG Wei;WANG Jingsong;GUO Ya’nan;GAO Le;WANG Lingling;CHEN Can;WANG Jianlin;WANG Jiandong;MA Ke;LI Jidong(Institute of Animal Science,Ningxia Academy of Agriculture and Forestry,Yinchuan 750002,China;School of Animal Science and Technology,Ningxia University,Yinchuan 750021,China;Ningxia Tongxin County Science and Technology Service Center,Tongxin 751300,China)

机构地区：[1]宁夏农林科学院动物科学研究所,银川750002 [2]宁夏大学动物科技学院,银川750021 [3]宁夏同心县科技服务中心,同心751300

出　　处：《畜牧兽医学报》2024年第7期3261-3266,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：宁夏回族自治区重点研发计划项目(2022BBF03024)。

摘　　要：旨在调查宁夏地区犊牛腹泻性大肠杆菌(Escherichia coli,E.coli)流行情况及毒力因子流行特点。对宁夏地区共采集332份腹泻犊牛直肠粪便进行病原分离,利用PCR方法对156株大肠杆菌分离株进行13种相关毒力基因检测。结果共分离出156株大肠杆菌(46.99%),共检出136株含有毒力因子的大肠杆菌,表明目前宁夏地区腹泻犊牛大肠杆菌阳性率较高,毒力因子组合复杂,优势毒力因子为irp2。The aim of this study was to investigate the prevalence and virulence factors of Escherichia coli(E.coli)in calves in Ningxia region.A total of 332 samples of diarrhea calf rectal feces were collected in Ningxia region for pathogen isolation,and 156 strains of E.coli were detected for 13 related virulence genes using PCR method.The results show that a total of 156 strains(46.99%)of E.coli were isolated,and a total of 136 strains containing virulence factors were detected.This indicates that the current positive rate of E.coli in diarrhea calves in Ningxia is relatively high,and the combination of virulence factors is complex,with irp2 being the dominant virulence factor.

关 键 词：犊牛 腹泻性 大肠杆菌 毒力因子 

分 类 号：S852.612[农业科学—基础兽医学]