基于数据挖掘技术探讨醒脑开窍针刺治疗缺血性脑血管病的作用机制  

Exploring Mechanism of Xingnao Kaiqiao Needling for Treatment of ICVD Based on Data Mining Technology

作　　者：赵晓慧 李德辉 高维娟 ZHAO Xiaohui;LI Dehui;GAO Weijuan(Hebei University of Chinese Medicine,Shijiazhuang 050091,China;The First Affiliated Hospital of Hebei University of Chinese Medicine,Shijiazhuang 050011,China;Hebei Key Laboratory of Chinese Medicine Research on Cardio-Cerebrovascular Disease,Shijiazhuang 050011,China)

机构地区：[1]河北中医药大学,河北石家庄050091 [2]河北中医药大学第一附属医院,河北石家庄050011 [3]河北省心血管疾病中医研究重点实验室,河北石家庄050011

出　　处：《针灸临床杂志》2024年第7期73-79,共7页Journal of Clinical Acupuncture and Moxibustion

基　　金：国家自然科学基金,编号:81603412;河北省重点研发计划,编号:18277731D;河北省中医药管理局科研计划,编号:2017163、2019008;河北中医学院科研能力提升一般项目,编号:KTY2019009;河北省“三三三人才工程”项目,编号:A202002008;河北省研究生创新基金,编号:XCXZZSS2022011、XCXZZSS2023017和XCXZZSS2023027;河北省心肺血管疾病中医研究重点实验室和河北省肝肾疾病及综合征中西医结合研究重点实验室,编号:A201902。

摘　　要：目的:参照网络药理学方法挖掘醒脑开窍针刺治疗缺血性脑血管病(ICVD)的核心穴位,并预测核心穴位治疗ICVD的作用机制。方法:检索以醒脑开窍法为原则的针刺治疗ICVD的相关文献,使用Excel软件建立针灸处方数据库,采用IBM SPSS Modeler软件对穴位数据进行关联规则分析以获取核心用穴;运用Cytoscape软件将穴位-靶点-疾病互作网络可视化;运用String数据库筛选核心靶点,构建蛋白质互作网络;通过DAVID在线分析工具对关键靶点进行功能及通路富集分析,并运用Bioinformatics免费在线平台对富集结果进行可视化。结果:共纳入文献70篇,相关腧穴17个;通过关联规则分析得出,醒脑开窍针灸的核心用穴为:人中、内关、三阴交、极泉、尺泽及委中;通过穴位-靶点-疾病网络可以看出作用于核心用穴可使TIMP-1、BDNF及VEGF等33个表达因子上调,使MMP-9、NSE、IL-6及TNF-α等49个表达因子下调;通过蛋白质互作网络得出核心用穴的核心作用靶点包括AKT1、IL6、TNF及IL1B等17个;通过富集分析得出,醒脑开窍法治疗ICVD可能是作用于脂质与动脉粥样硬化及流体剪切力和动脉粥样硬化等信号通路实现的。结论:人中、内关、三阴交、极泉、尺泽和委中为醒脑开窍法治疗ICVD的核心用穴,核心用穴通过调控AKT1、IL6、TNF与IL1B等核心靶点,参与脂质与动脉粥样硬化、流体剪切力和动脉粥样硬化等信号通路治疗ICVD。Objective:To explore the core acupoints of Xingnao Kaiqiao needling in treatment of ischemic cerebrovascular disease(ICVD),and to predict its mechanism in treating ICVD based on network pharmacology.Methods:The relevant literature of acupuncture treatment for ICVD based on the principle of Xingnao Kaiqiao,which means awakening brain and opening orifices,were retrieved to establish acupuncture prescription by using Excel software.The association rule analysis of acupoint data was carried out to obtain the core points with IBM SPSS Modeler software.The acupoint-target-disease interaction network was visualized through using cytoscape software.String database was used to screen out core targets and build protein-protein interaction(PPI)network.The functional and pathway enrichment analysis of key targets was conducted through DAVID online analysis tool,and the enrichment results were visualized by applying the Bioinformatics free online platform.Results:A total of 70 pieces of literature were included,involving 17 acupoints.Through association rule analysis,the core acupoints of Xingnao Kaiqiao needling included Renzhong(DU26),Neiguan(PC6),Sanyinjiao(SP6),Jiquan(HT1),Chize(LU5)and Weizhong(BL40).According to the acupoint-target-disease network,33 expression factors including TIMP-1,BDNF and VEGF were up-regulated,and 49 expression factors including MMP-9,NSE,IL-6 and TNF-αwere down-regulated after core acupoints intervention.Through the PPI network,17 core targets of core acupoints were obtained,including AKT1,IL6,TNF and IL1B.The enrichment analysis showed that the treatment of ICVD by Xingnao Kaiqiao needling may be achieved by working on the lipid and atherosclerosis signaling pathway and the fluid shear force and atherosclerosis signaling pathway.Conclusion:DU26,PC6,SP6,HT1,LU5 and BL40 are the core acupoints for the treatment of ICVD by Xingnao Kaiqiao needling.The core acupoints are involved in the treatment of ICVD by regulating the core targets including AKT1,IL6,TNF,IL1B and other signaling pathways includ

关 键 词：网络药理学 醒脑开窍 针刺 核心用穴 缺血性脑血管病 

分 类 号：R246.6[医药卫生—针灸推拿学]