响应性透明质酸胶束的合成与性能分析  

Synthesis and properties of responsive hyaluronic acid micelles

作　　者：刘超凡 廖盛龙 仲舒颖 林英[1] LIU Chaofan;LIAO Shenglong;ZHONG Shuying;LIN Ying(School of Chemical and Environmental Engineering,Anhui Polytechnic University,Wuhu 241000,China)

机构地区：[1]安徽工程大学化学与环境工程学院,安徽芜湖241000

出　　处：《中国塑料》2024年第7期25-31,共7页China Plastics

基　　金：安徽省重点研究与开发计划项目(2022h11020005);安徽省高校优秀科研创新团队项目(2023AH010012)。

摘　　要：透明质酸(HA)是一种具有癌细胞靶向性的天然多糖,基于特殊功能基团可用于构建病灶环境敏感的高分子纳米载体。本文利用可逆加成⁃断裂链转移(RAFT)聚合可控合成了3⁃丙烯酰胺基苯硼酸与乙烯基吡咯烷酮的嵌段共聚物,通过硼酸酯键与HA自组装形成了纳米胶束。调节两种高分子的投料浓度及优化共聚物的链长比例,能够实现对胶束水合粒径的调控。共聚物浓度为7.5 mg/mL、聚合度为40且嵌段分子量比为2时,组装粒子粒径可以控制在73.3~163.9 nm,有利于体内长循环及病灶渗透。研究表明,该纳米粒子具有良好的pH、糖和盐响应性。合成了多重响应的癌细胞靶向聚合物纳米胶束,具有用作抗肿瘤活性药物及探针载体的潜能。Hyaluronic acid(HA)is a natural polysaccharide with cancer cell targeting properties,and it can be used to construct polymeric nanocarriers sensitive to lesion environments based on special functional groups.Herein,the reversible addition⁃fragmentation chain transfer(RAFT)polymerization was used to synthesize a block copolymer of 3⁃acryl⁃amide phenylboronic acid and vinyl pyrrolidone controllably.This block copolymer was self⁃assembled with hyaluronic acid through the borate ester bond to form nano⁃micelles.Adjusting the feeding concentration of two types of polymers and optimizing the chain length ratio of copolymers can obtain the regulation of micellar hydrodynamic diameter.When the concentration of copolymer was 7.5 mg/mL,the degree of polymerization was 40 and the molecular weight ratio of block was 2.The obtained particle size could be tunable from 73.3 to 163.9 nm,which is advantageous to long circulation in vivo and lesion penetration.The findings indicated that the nanoparticles had suitable pH,sugar,and salt responsiveness.In summary,a multi⁃responsive cancer cell targeting polymer nano⁃micelle has been synthesized in this study,and it exhibits great potential for applications as an antitumor drug and probe carrier.

关 键 词：透明质酸 3⁃丙烯酰胺基苯硼酸 嵌段共聚 响应性 纳米胶束 

分 类 号：TQ320[化学工程—合成树脂塑料工业]