基于年龄-龄期两性生命表的甲氨基阿维菌素苯甲酸盐对白背飞虱的亚致死效应分析  

Analysis of the sublethal effects of emamectin benzoate on Sogatella furcifera(Hemiptera:Delphacidae)based on the age-stage,two-sex life table

作　　者：章冰川 廖琪 张世燕 何树林 乔梁[1,2] 周操 ZHANG Bing-Chuan;LIAO Qi;ZHANG Shi-Yan;HE Shu-Lin;QIAO Liang;ZHOU Cao(College of Life Sciences,Chongqing Normal University,Chongqing 401331,China;Chongqing Key Laboratory of Vector Control and Utilization,Institute of Entomology and Molecular Biology,Chongqing Normal University,Chongqing 401331,China)

机构地区：[1]重庆师范大学生命科学学院,重庆401331 [2]重庆师范大学昆虫与分子生物学研究所,媒介生物控制和利用重庆市重点实验室,重庆401331

出　　处：《昆虫学报》2024年第6期753-765,共13页Acta Entomologica Sinica

基　　金：重庆市教委科学技术研究项目(KJQN202200533,KJQN202200532,KJZD-K202200507);重庆市博士“直通车”科研项目(CSTB2022BSXM-JCX0067);重庆市自然科学基金面上项目(cstc2021jcyj-msxmX0235)。

摘　　要：【目的】甲氨基阿维菌素苯甲酸盐是高效治理鳞翅目(Lepidoptera)害虫的新型抗生素类生物源杀虫剂。本研究旨在探究甲氨基阿维菌素苯甲酸盐对水稻害虫白背飞虱Sogatella furcifera的亚致死效应。【方法】采用稻茎浸渍法以不同浓度甲氨基阿维菌素苯甲酸盐处理的水稻苗饲养白背飞虱3龄若虫,72 h后计算甲氨基阿维菌素苯甲酸盐对白背飞虱3龄若虫的LC_(10),LC_(25)和LC_(50)值;采用稻茎浸渍法以LC_(10),LC_(25)和LC_(50)浓度甲氨基阿维菌素苯甲酸盐处理的水稻苗饲养白背飞虱3龄若虫48 h,测定F0代雌成虫寿命和单雌产卵量;统计F_(1)代卵历期、1-5龄若虫历期、成虫寿命、成虫前历期、总发育历期、成虫产卵前期、成虫总产卵前期和单雌产卵量,构建年龄-龄期两性生命表;利用Timing-MSChart软件预测白背飞虱未来60 d种群动态。【结果】处理72 h时甲氨基阿维菌素苯甲酸盐对白背飞虱3龄若虫的LC_(50),LC_(25)和LC_(10)值分别为0.831,0.222和0.068 mg/L。与对照组相比,LC_(10),LC_(25)和LC_(50)浓度甲氨基阿维菌素苯甲酸盐处理使白背飞虱F0代雌成虫平均寿命分别缩短了7.19%,24.81%和34.21%,单雌产卵量分别减少22.24%,31.22%和41.53%;LC_(25)浓度甲氨基阿维菌素苯甲酸盐处理显著延长了F_(1)代5龄雌若虫历期,LC_(50)浓度甲氨基阿维菌素苯甲酸盐处理显著延长了F_(1)代雄成虫寿命和总发育历期,LC_(25)和LC_(50)浓度甲氨基阿维菌素苯甲酸盐处理显著延长了F_(1)代成虫产卵前期和总产卵前期,且显著降低了内禀增长率(r)和周限增长率(λ);LC_(50)浓度甲氨基阿维菌素苯甲酸盐处理下F_(1)代净增殖率(R_(0))显著降低,LC_(25)浓度甲氨基阿维菌素苯甲酸盐处理下F_(1)代平均世代周期(T)显著延长;LC_(10),LC_(25)和LC_(50)这3个亚致死浓度甲氨基阿维菌素苯甲酸盐处理明显抑制了白背飞虱种群增长。【结论】亚致死浓度甲氨基阿维菌�【Aim】Emamectin benzoate(EMB)is a novel antibiotic bio-derived insecticide and highly efficient in controlling lepidopteran pests.This study aims to investigate the sublethal effects of EMB on the rice pest of Hemiptera,Sogatella furcifera.【Methods】Using rice stem-dipping method,we first determined the LC_(10),LC_(25) and LC_(50) values of EMB against the 3rd instar nymphs of S.furcifera reared for 72 h on the rice seedlings treated with EMB at different concentrations.The 3rd instar nymphs of S.furcifera were reared on rice seedlings treated with EMB at the LC_(10),LC_(25) and LC_(50) concentrations for 48 h through the rice stem-dipping method.The female adult longevity and the number of eggs laid per female of the F 0 generation were determined.The duration of egg and the 1st-5th instar nymphs,adult longevity,pre-adult duration,total developmental duration,adult pre-oviposition period,adult total pre-oviposition period,and the number of eggs laid per female of the F_(1) generation were counted to construct the age-stage,two-sex life table.The population dynamics of S.furcifera were predicted in 60 d by using Timing-MSChart software.【Results】The LC_(50),LC_(25) and LC_(10) values of EMB against the 3rd instar nymphs of S.furcifera were 0.831,0.222 and 0.068 mg/L,respectively,at 72 h after treatment.In the F 0 generation,S.furcifera female adults treated with EMB at the concentrations of LC_(10),LC_(25) and LC_(50) had reduced average longevity by 7.19%,24.81%and 34.21%,and decreased number of eggs laid per female by 22.24%,31.22%and 41.53%,respectively,compared with the control group.EMB at the LC_(25) concentration significantly prolonged the 5th instar female nymphal duration of the F_(1) generation and EMB at the LC_(50) concentration significantly prolonged the male adult longevity and total developmental duration of the F_(1) generation compared with the control group.EMB at the LC_(25) and LC_(50) concentrations significantly prolonged the adult pre-oviposition period and total pre-oviposition

关 键 词：白背飞虱 甲氨基阿维菌素苯甲酸盐 亚致死效应 年龄-龄期两性生命表 繁殖力 

分 类 号：Q965.9[生物学—昆虫学]